English

Marcela Carlota Nery,Fernanda Carlota Nery,Raquel Maria De Oliveira Pires

doi:10.14393/bj-v31n3a2015-22527

Abstract

The use of tetrazolium test is important in the evaluation of seeds lot quality and it has been adopted for vigor and viability identification for several species. The interest on the production of oil radish is increasing since the seeds were considered a good source of oil for biofuel production. The development of the tetrazolium test methodology for seeds of this species can improve the seed quality control process, and additionally will provide information for the characterization of remaining seeds (dead or dormant) in the germination tests. To verify the ideal conditions to tetrazolium test was conducted two experiments. At the first, oil radish seeds cultivar CATI AL-1000, lots from 2001 and 2006 were submitted to imbibition between paper in water for 6 hours. After the longitudinal cut in the longest direction, the seeds were immersed in the tetrazolium solution at the concentrations of 0,075%, 0,5% and 1% at 25°C for 3h, 12h and 18h. In the second experiment, oil radish seeds cultivar CATI AL-1000 lot from 2005 and IPR 116 cultivar, lots from 2004 and 2005 were immersed in the concentrations of ,1%; 0,2%; 0,3% and 0,4% of tetrazolium solution for 12 hours at a 25°C, 30°C, 35°C and 40°C. At the first experiment was observed the necessity of test intermediate concentrations between 0,075% and 0,5%, since with 0,075% the seeds stained weakly and with 0,5% the test results, were overestimated. In the second experiment was observed that the 0,3% concentration at 30°C can be recommended for the utilization of tetrazolium test to evaluation of oil radish seeds viability.

Highlights

The species Raphanus sativus L. is a plant from the Brassicaceae family with a relatively easy cultivation by the natural hardiness and tolerance to the adverse conditions besides the ability to develop relatively well in soils with low fertility and slightly acids
The research was divided in two experiments: in the experiment one were used two lots of oil radish seeds, cultivar CATI AL-1000, lot 1 and lot 2, from the region of Varginha, MG
The moisture content of lots had the average of 6,5% in the occasion of realization of the test, for lot 1 of 6,0% e lot 2 of 7,0%, what indicates uniformity of lots in relation to this factor what can interfere in the pre-conditioning period

Summary

Introduction

The species Raphanus sativus L. is a plant from the Brassicaceae family with a relatively easy cultivation by the natural hardiness and tolerance to the adverse conditions besides the ability to develop relatively well in soils with low fertility and slightly acids.Is a plant very used in the crop rotation and green manure due to the higher potential of nutrients cycling. The species Raphanus sativus L. is a plant from the Brassicaceae family with a relatively easy cultivation by the natural hardiness and tolerance to the adverse conditions besides the ability to develop relatively well in soils with low fertility and slightly acids. The seeds have significant content of oil with a relative easy extraction, what can becomes important to the rural producers for the production of oil in the Biodiesel Program (SILVA, 2007). The potential for the oil extraction until today, it was not much explored, both by the low technology for the production of grains or by the availability of quality seeds. A big challenge for the research institutions and companies producers of seeds, has been the evaluation of lots viability, what allows the selection of seeds on sowing. For species like R. sativus L., there are not enough researches in this area

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