Abstract
The evaluation and comparison of the staining effect of pomegranate (Punica granatum) flower extract (dye solution) on histological sections of rat testis and ovary provides a simple, quick technique for the visualisation of both cells. In the developed procedure, this work shows the staining effect of P. granatum flower extract used at different pH of dye bath and temperatures on the histological sections of rat testis and ovary. A 20% stock solution of P. granatum flower extract was prepared by dissolving 20 g of the dye in 100 mL ethanol at room temperature for several hours. After 24 h, the deep-red coloured solution was filtered (0.45 µm filter) to remove any undissolved dye. This stock solution was stored at room temperature and each day a 5 mL sample was taken and adjusted to different pH with dilute ammonia (NH3) using pH meter. Best staining effects conditions were obtained by testing the different pH of dye (1-2, 4-5) and temperature. The testis and ovary are stained in different colors; lamina propria section and spermatogonia cells of testis were more purple than the same sections of ovary at room temperature and pH of 1-2 dye bath. Therefore, the developed method has been applied successfully for the visualization of rat testis and not the ovarian tissue. Key words: Histological section, ovarian, pomegranate, Punica granatum, testis.
Highlights
This work shows the staining effect of P. granatum flower extract used at different pH of dye bath and temperatures on the histological sections of rat testis and ovary
The testis and ovary are stained in different colors; lamina propria section and spermatogonia cells of testis were more purple than the same sections of ovary at room temperature and pH of 1-2 dye bath
Testis and ovarian tissues were stained in pomegranate flower extract
Summary
The flowering habit of pomegranate is influenced by the climatic condition of the geographical region where it is grown (Babu, 2010) Plants and their extracts are used for medicinal purpose both for the prevention and treatment of human diseases (Zeliha et al, 2016). Six known compounds were identified by comparing their spectral data with values reported in the literature as ellagic acid, 3, 3’,4’- tri-O-methylellagic acid, ethyl brevifolin carboxylate, urolic acid, maslinic acid, and daucosterol (Wang et al, 2010; Mahmoud et al, 1994) According to their sources, dyes are divided into two classes as natural and synthetic (Nagar et al, 2005). It is used to demonstrate general tissue structures (Avwioro, 2002) This is the first study where pomegranate flower extract is used on testis and ovarian tissues. The aim is to elucidate the differences that occur when we dye testis and ovarian tissues under the same conditions, to reveal the positive and negative results of dyes and to direct future studies
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