Abstract
The morphogenic potential of nodal explants of Piper aduncum and Piper hispidinervum (Piperaceae) was investigated and a protocol for rapid micropropagation is described. An experiment based on the saline formulation of Murashige and Skoog (MS) and Wood Plant medium (WPM) combined with different N6-benzylaminopurine (BAP) and 1-naphthalene acetic acid (NAA) concentrations was evaluated. After determining the optimal concentration of growth regulators, the multiplication rates for the species, which were distributed in five subcultures, was assessed, and the number of plantlets produced in this period was recorded. After assessing the plants from all five subcultures, plantlets with well-developed root and shoot systems were transferred to pots containing substrate for acclimatization. The culture of nodal segments of P. aduncum and P. hispidinervum on hormone-free medium was shown to be a suitable method for micropropagation due to the high multiplication rate and good plant development. The use of BAP or BAP + NAA resulted to formation of vitrified multiple shoots and callus formation at the base of the microcuttings. Even at concentrations lower than 1 mg L-1, the use of BAP resulted in vitrified multiple shoot and callus formation, without significantly improving the multiplication rates. For both species, the first subculture resulted in the greatest number of axillary buds, and mainly for P. hispidinervum, the MS medium was the most appropriate for in vitro multiplication of microcuttings. The species showed 100% root formation, and acclimatization of plants from the fifth subculture in a greenhouse resulted in 100% survival. Key words: Spiked pepper, long pepper, micropropagation, dillapiol, safrole, morphogenesis, organogenesis
Highlights
Piper aduncum commonly known as spiked pepper, andPiper hispidinervum, known as long pepper, are plant species found in the Amazon Region that have been generating economic interest due to their phytochemical properties.Belonging to the Piperaceae family, these shrub-like species are a natural resource of great commercial value, due to their respective secondary compounds, dillapiol and safrole, which are the major compounds in the essential oils of these plants
After determining the optimal concentration of growth regulators, the multiplication rates for the species, which were distributed in five subcultures, was assessed, and the number of plantlets produced in this period was recorded
When the percentage of explants with multiple shoots and callus was assessed, it was observed that the addition of BAP or BAP + naphthalene acetic acid (NAA) to the culture medium caused the formation of multiple shoots and calli in the explants, regardless of the saline formulation used (Figure 1-B)
Summary
Tatiane Loureiro da Silva, Talita Aparecida Balzon and Jonny Everson ScherwinskiPereira3*. The culture of nodal segments of P. aduncum and P. hispidinervum on hormone-free medium was shown to be a suitable method for micropropagation due to the high multiplication rate and good plant development. The use of BAP or BAP + NAA resulted to formation of vitrified multiple shoots and callus formation at the base of the microcuttings. Even at concentrations lower than 1 mg L1, the use of BAP resulted in vitrified multiple shoot and callus formation, without significantly improving the multiplication rates. For both species, the first subculture resulted in the greatest number of axillary buds, and mainly for P. hispidinervum, the MS medium was the most appropriate for in vitro multiplication of microcuttings.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
