English

Q He Y,Q Chen H,Y Liu X,K Ma X,Kong J,X Zhang C

doi:10.5897/ajb09.1715

Abstract

  To screen the genes controlling the fecundity traits in goats, a DDRT-PCR technique was applied. We found a new EST which highly expressed in Chinese native prolific goat breed, Haimen goats. There exists a difference of EST expression level between the prolific and non-prolific goat breed, indicating EST might associate to fecundity in goats. A full-length cDNA with 2253 base pairs was obtained by the 3’- and 5’-RACE method based on the EST sequence encoding a protein segment of 201 amino acid residues. Tissue specific distribution and sequence analysis implicated the likely involvement of EST in the regulation of the hormones related to fecundity.   Key words: Goat, expressed sequence tag, reproduction.

Highlights

The events of the goat estrous cycle, just like other mammalians, are largely controlled by the relationship between the hypothalamic releasing hormones, gonadotropins and ovarian hormones, so-called hypothalamushypophyseal axis modulate
We found a new expressed sequence tag (EST) which highly expressed in Chinese native prolific goat breed, Haimen goats
There exists a difference of EST expression level between the prolific and non-prolific goat breed, indicating EST might associate to fecundity in goats

Summary

Introduction

The events of the goat estrous cycle, just like other mammalians, are largely controlled by the relationship between the hypothalamic releasing hormones, gonadotropins and ovarian hormones, so-called hypothalamushypophyseal axis modulate. To screen the genes controlling the fecundity traits in goats, a DDRT-PCR technique was applied. There exists a difference of EST expression level between the prolific and non-prolific goat breed, indicating EST might associate to fecundity in goats. Tissue specific distribution and sequence analysis implicated the likely involvement of EST in the regulation of the hormones related to fecundity.

Results

Conclusion