Abstract
In this study, the effects of supercritical CO2 extraction and macroporous adsorbent resin on separation and purification of apigenin were compared. A better separation and purification macroporous adsorbent resin was selected according to static adsorption and desorption results. Silica gel column chromatography and LSA-10 macroporous adsorbent resin performed better in the separation and purification procedure. The purity of apigenin could reach 92.8% after separation and purification. The optimal supercritical CO2 extraction conditions of apigenin from Viola yedoensis makino are solid to liquid ratio: 5:3, extraction pressure: 20 MPa, extraction temperature: 55°C, extraction time: 2 h, separation vessel temperature: 55°C, pressure: 9 Mpa, extraction vessel temperature: 40°C and pressure：5 MPa. Supercritical CO2 extraction had a great effect on the separation and purification of apigenin from V. yedoensis makino; the purity could reach 97.6%. Assaying apigenin by high performance liquid chromatography (HPLC) showed that the sample volume and peak area of the response have a good linear relationship, r = 0.9996, between 1.58 to 15.80. The results indicated that the procedure of separation and purification of apigenin from V. yedoensis by supercritical CO2 extraction, which is better than that of macroporous adsorbent resin, is suitable for the separation and purification of high purity apigenin. Key words: Extraction, purification, macroporous adsorbent resin, supercritical CO2 extraction.
Highlights
In this study, the effects of supercritical CO2 extraction and macroporous adsorbent resin on separation and purification of apigenin were compared
The results indicated that the procedure of separation and purification of apigenin from V. yedoensis by supercritical CO2 extraction, which is better than that of macroporous adsorbent resin, is suitable for the separation and purification of high purity apigenin
The methods used for apigenin extraction were mainly by maceration or reflux extraction with methanol, ethanol and other organic solvents (Peng, 2009; Shen et al, 2010; Jiang et al, 2013 ), while the ultrasonic- and microwave-assisted extractions were applied by some investigators (Liu et al, 2010)
Summary
The effects of supercritical CO2 extraction and macroporous adsorbent resin on separation and purification of apigenin were compared. The results indicated that the procedure of separation and purification of apigenin from V. yedoensis by supercritical CO2 extraction, which is better than that of macroporous adsorbent resin, is suitable for the separation and purification of high purity apigenin. “Materia Medica justice" recognizes that the plant provided a useful generic drug against carbuncle swelling and boil poison (Drug administration department of the People's Republic of China, 2000; Zhuang, 2002; Wen et al, 2006) It was reported in the current paper that macroporous adsorbent resin or a silica gel extraction column was used in the separation and purification of the apigenin (Zhu et al, 2005; Dawa et al, 2008; Zhu et al, 2005; Li et al, 2007; Zhang et al, 2004). The majority of apigenin in the violaceae viola exist as various kinds of glycosides
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