Abstract
Dendrobium wangliangii is seriously threatened because of anthropogenic and environmental pressures. A protocol with seed asymbiotic germination was developed to conserve the orchid in vitro. The germination rate was 92% under 16/8 h (light/dark) photoperiod after 240 days from hand-pollination in a modified medium. Polyembryony was founded in D. wangliangii, with a percentage of 0.87%. Half MS promoted the protocorms proliferation well, with the protocorms differentiated at the frequency of 28.79% when cultured on half MS, supplemented with 2 mg L-1 6-benzylaminopurine (BA) and 0.1 mg L-1naphthaleneacetic acid (NAA). Additives, especially 100 ml L-1 coconut milk (CM), improved the protocorm differentiation greatly but led to a lethality of 49.92%. The pretreatment of 1 mg L-1 GA3 for 15 days, after inoculated to the medium amended with 2 mg L-1 BA, 0.1 mg L-1 NAA and 100 ml L-1 CM, increased the differentiation to 54.68%. The combination of 2 mg L-1 BA, 0.1 mg L-1 NAA, and 100 ml L-1 CM also showed an efficient role in adventitious bud multiplication. CM effectively controlled the albino phenotype. In addition, inflorescence induction (100%) was produced with 2 mg L-1 1-phenyl-3-(1, 2, 3-thiadiazol-5-yl)-urea (TDZ) and the best flower development was obtained with 0.3 mg L-1 paclobutrazol (PP333), and 0.5 mg L-1 NAA. Key words: Dendrobium wangliangii, micropropogation, asymbiotic germination, adventitious buds multiplication, polyembryony, in vitro flowering.
Highlights
Dendrobium wangliangii is one of the largest genera in the Orchidaceae comprising approximately 1,100 species (Puchooa et al, 2004)
The purpose of this study is to investigate the factors that are relative with the seed germination, to develop a highly efficient micropropagation protocol, and to assess the effects of hormones on the in vitro flowering
As in the case of D. wangliangii, seeds are light yellow-white and approximately 300 μm long with the embryos accounting for half of the total length (Figure 1a)
Summary
Dendrobium wangliangii is one of the largest genera in the Orchidaceae comprising approximately 1,100 species (Puchooa et al, 2004). About 80 species of Dendrobium are known from China, most occurring in tropical and subtropical regions of the south (Hu et al, 2008). Dendrobium is one of the most famous traditional herbs in China. H. Jin is a newly discovered and described species with stems closely attached to the host’s bark, making it distinguishable from other species (Hu et al, 2008). The mucopolysaccharide-rich fleshy stem is similar to two species with important morphological and medicinal properties, Dendrobium officinale Kimura and Migo, and Dendrobium huoshanense C. J. Cheng, suggesting D. wangliangii probably has high medicinal value which is confirmed by the fact that the locals collect
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