Abstract
Beclardia macrostachya is one of the rarest orchids in Mauritius. In vitro techniques are being used for mass propagation of this orchid for subsequent restoration programs. Successful asymbiotic germination of Beclardia macrostachya was obtained through embryo rescue under in vitro conditions. Modified half MS medium supplemented with 10% coconut milk was used as basal culture medium and the effect of plant growth regulators at different concentrations on embryo development was assessed through qualitative and quantitative parameters. Diameter of embryos, length of protocorm-like bodies (PLBs) and length of developing shoots were calculated using digital photography. Maximal growth was obtained in treatments without any plant growth regulators and with 0.5 mg/L N6-Benzylaminopurine (BAP). Higher levels of Thidiazuron/TDZ (0.3 mg/L) and BAP (1.0 mg/L) though they stimulated embryo development faster, yielded higher level of necrosis later. The results also suggest that plant growth regulator treatments that stimulate fastest embryo development from immature embryos/ovules need not be reliable for further development to PLB and plantlet regeneration.
Highlights
Abstract invades embryo cells, have their hyphae later destroyed by phytoalexins and enzymes and are used as resources for embryo growth
O Successful asymbiotic germination of Beclardia macrostachya was obtained through e embryo rescue under in vitro conditions. s Modified half MS medium supplemented with u 10% coconut milk was used as basal culture l medium and the effect of plant growth regulaia tors at different concentrations on embryo development was assessed through qualitative c and quantitative parameters
The results suggest that plant o growth regulator treatments that stimulate fastest embryo development from immature
Summary
The culture medium used was a modified half-strength Murashige and Skoog’s10 medi-. The capsules were carefully cut open, and small bits (~2 cm2) of whitish mass of embryos along with the placental tissue were sliced and placed on the culture media. A minimal of 50 purposes clonal propagation is preferentially um,[11] as suggested by Chen et al The basal culture tubes for each treatment was inoculatused because certain characteristics, such as culture media contained 1⁄2 strength macro and ed and readings were taken on a weekly basis. Micro-elements of MS supplemented with Each culture tubes that showed fungal infec-. Qualitative and Quantitative data were collected to assess and compare growth under calculate the percentage of cultures that sur- different PGR treatments. After 3 months ten test-tubes selected, that showed uniform growth of PLBs, for each treatment and were used for digital photogra-
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