Abstract
The content of flavonoids and phenolic acids in the extract of bamboo leaves (EBL) was determined using near-infrared spectroscopy. Optimal partial least squares models and least squares-support vector machine models were developed to enable rapid and non-destructive validation based on EBL contents as determined by photocolorimetric methods and high performance liquid chromatography. The least squares-support vector machine models performed slightly better than the partial least squares models. For least squares-support vector machine models, the correlation coefficients (R) and root mean square errors (RMSE) of calibration and validation were R(cal)= 0.9998, RMSE(cal)= 0.05, R(val)= 0.9418 and RMSE(val)= 3.91 for total flavonoids (TF) and R(cal)= 0.9778, RMSE(cal)= 2.49, R(val)= 0.9535, and RMSE(val)= 3.61 for total phenolic acids (TP). For optimal partial least squares models, the corresponding values were 0.9962, 1.12, 0.9124 and 4.97 for TF and 0.9966, 0.98, 0.9325 and 4.33 for TP. The results indicated that near-infrared spectroscopy with least squares-support vector machine could be utilized as a precise method for the determination of flavonoids and phenolic acids in EBL.
Highlights
Bamboo is a giant, woody grass with has a tropical and subtropical distribution and represents an important commodity
The results indicated that near-infrared spectroscopy with least squares-support vector machine could be utilized as a precise method for the determination of flavonoids and phenolic acids in extract of bamboo leaves (EBL)
In order to determine whether one pure constituent or the sum of four constituents could be determined by near-infrared spectroscopy, reversed phase high performance liquid chromatography (HPLC) was used to separate and quantify the main flavonoids and phenolic acids against known standards (Sigma-Aldrich Co., Ltd., USA)
Summary
Woody grass with has a tropical and subtropical (cosmopolitan) distribution and represents an important commodity. Bamboo leaves have been used in traditional Chinese medicine for treating fever and detoxification for over 1000 years. Some biologically active components in bamboo leaves and their potential health benefits have been widely studied. Extract of bamboo leaves (EBL) is a polyphenol-rich preparation. Animal experiments and clinical trials have confirmed that EBL has medicinal properties, such as antioxidant, anti-aging, antibiosis, antiviral and neuroprotective potential (Zhang, 1995). For this reason, EBL has been added to foods, beverages, wine, cosmetics and animal feed and has a large pros-pective market in Asia. As with the production of other plant extracts, the content of these bioactive components in EBL preparations can be
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