Abstract
To investigate the effect of exogenous Nitric oxide (NO) on submerged plants,Hydrilla verticillata (L.f.) Royle was treated with 25 - 400 μΜ sodium nitroprusside (SNP, a NO donor) for 0.5 - 4 d. The alterations in plant growth, H2O2, total chlorophyll and malondialdehyde (MDA) content and the antioxidant response were assayed. The results showed that 25 - 100 μΜ SNP increased the plant growth and total chlorophyll content and reduced the level of H2O2. However, an increase in H2O2 and MDA content and a decrease in total chlorophyll were detected in plants exposed to 200 - 400 μΜ SNP. The activity of catalase and peroxidase decreased in plants exposed to 25 - 400 μΜ SNP; and for superoxide dismutase, its activity was suppressed by the 25 and 50 μM SNP. The content of ascorbic acid and dehydroascorbate were also determined. The results provided the evidence that 25 - 100 μM SNP increased the growth of H. verticillata by alleviating the oxidative stress and 200 - 400 μM SNP might enhance the oxidative stress in plant cell. The results suggested that NO has dual role on the growth and ROS metabolism ofHydrilla verticillata (L.f.) Royle. Key words: Nitric oxide, Hydrilla verticillata (L.f.) Royle, chlorophyll, growth, malondialdehyde.
Highlights
Nitric oxide (NO) has been considered as an important second messenger in organism
These demonstrated that compared with control plants (0 μM) relative growth rate increased in plants treated with 25 - 100 μM sodium nitroprusside (SNP) but was inhibited at 200 and 400 μM SNP
Compared with control plants (0μM SNP), H2O2 levels decreased in plants exposed to 25, 50 and 100 μM SNP, while it increased in plants treated with 200 and 400 μM SNP
Summary
Nitric oxide (NO) has been considered as an important second messenger in organism. In animals, NO can be produced by the activity of NO synthases (NOS) and involves in many physiological processes including vasorelaxation, platelet inhibition, neurotransmission, cytotoxicity, and immunoregulation (Brunori et al, 1999). By reacting with many targets (Wink et al, 1993; Beligni and Lamattina, 2001; Wendehenne et al, 2001), NO can involve in the regulation of physiological responses including photomorphogenesis, seed germination, deetiolation, hypocotyl elongation (Leshem and Haramaty, 1996; Beligni and Lamattina, 2001), organ maturation and senescence (Leshem, 1996; Leshem et al, 1998; Tu et al, 2003). NO is involved in many processes as antioxidant agent in plants in responses to abiotic stresses from heavy metals and UV-B-radiation (Beligni and Lamattina, 1999a; García-Mata and Lamattina, 2001; Uchida et al, 2002)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
