外源NO对镉胁迫下水稻幼苗抗氧化系统和微量元素积累的影响

Abstract

PDF HTML阅读 XML下载 导出引用 引用提醒 外源NO对镉胁迫下水稻幼苗抗氧化系统和微量元素积累的影响 DOI: 10.5846/stxb201111251802 作者: 作者单位: 杭州师范大学,杭州师范大学,杭州师范大学,杭州师范大学,杭州师范大学,杭州师范大学,杭州师范大学,杭州师范大学 作者简介: 通讯作者: 中图分类号: 基金项目: 国家自然科学基金资助项目(30800676;31171535);浙江省自然科学基金资助项目(Y3090114);浙江省重点科技创新团队基金资助(2010R50039);杭州师范大学生物科学国家特色专业本科生科技创新项目 The effect of exogenous nitric oxide on activities of antioxidant enzymes and microelements accumulation of two rice genotypes seedlings under cadmium stress Author: Affiliation: Hangzhou Normal University,Hangzhou Normal University,Hangzhou Normal University,Hangzhou Normal University,Hangzhou Normal University,Hangzhou Normal University,Hangzhou Normal University,Hangzhou Normal University Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:重金属污染已成为世界范围的主要问题之一,其中镉(Cd)毒害的范围最广。Cd污染影响植物的生长和发育。一氧化氮(NO)作为植物体内的一种活性信号因子,参与了植物对各种胁迫的应答。为了探讨外源NO对Cd胁迫下水稻苗期生理生化响应的调节作用,以粳稻和籼稻为材料,采用营养液水培的方法,研究外源NO供体硝普钠(SNP)对100 μmol/L Cd胁迫下2个水稻基因型(ZF802和ZH11)幼苗生长、抗氧化酶系统和微量元素吸收的影响。结果表明,1.5 mmol/L SNP 能明显缓解Cd胁迫对两种水稻幼苗生长的抑制作用,提高幼苗对Cd的耐性。尽管存在一定的基因型差异,但外源NO能使两个基因型叶片中的超氧化物歧化酶(SOD)和过氧化物酶(POD)活性下降,降低了叶片中丙二醛(MDA)含量,从而缓解Cd对水稻幼苗的毒害。另外,外源NO影响Cd胁迫下水稻幼苗地上部和根部Cd和微量元素的积累,这种影响的程度与水稻的品种有关,具有显著的基因型差异,且机制较复杂。 Abstract:Heavy metal pollution has become one of the major problems the world, with cadmium (Cd) the most widespread pollution. Cd is a heavy metal without biological functions and highly toxic to plants and animals. Its accumulation in soil is becoming a severe threat to crop production as well as human health. The presence of excessive amount of Cd may cause toxicity to plants, resulting in stunted growth, strong reduction in biomass production, damage of the photosynthetic apparatus, reduction in chlorophyll content and disturbance of mineral nutrition and carbohydrate metabolism. Nitric oxide (NO) is a bioactive gas and is an important signaling molecule with diverse physiological functions in plants. Recently, a lot of researchers found that NO plays an important role in mediating some biotic and abiotic stress-induced oxidative stresses in plant kingdom. To understand the regulative effect of exogenous nitric oxide on the physiological and biochemical responses of rice seedlings under cadmium stress, in the present study, two rice cultivars, ZF802 (indica) and ZH11 (japonica) grown in IRRI's nutrient solution were used to investigate the effects of exogenous nitric oxide (sodium nitroprusside as NO donor) on the growth of seedlings, activities of antioxidant enzymes (SOD and POD) and microelements accumulation in two rice genotypes under 100 μmol/L cadmium stress. The results showed that addition of 1.5 mmol/L exogenous SNP significantly alleviated the inhibitory effect of Cd2+ stress on the growth of rice seedlings. Exogenous SNP also decreased the activities of superoxide dismutase (SOD) and peroxidase (POD), and the contents of malondialdehyde (MDA) in the leaves of rice seedlings under Cd stress, despite of certain genotype difference, suggesting that SNP could alleviate oxidative damage of rice seedlings caused by Cd. In addition, exogenous NO supply affected Cd and microelements accumulation of the shoots and roots of rice seedlings under Cd stress. The effects had significant genotypic difference and complex mechanism. 参考文献 相似文献 引证文献