Abstract
A series of benzimidazole derivatives (5a-c) were synthesized by coupling 5-substituted 2-chloromethyl benzimidazole (3a-c) with 2-mercapto-N-methyl imidazole. The synthesized compounds were characterized by infra-red (IR), nuclear magnetic resonance (NMR) and elemental analyses. Further, the synthesized compounds were tested on plantlet production from protocorm like bodies (PLBs) sections of Oncidium. PLBs sections were cultured on half strength modified Murashige and Skoogs (MS) medium alone and also modified MS medium supplemented with the synthesized chloro, methyl and nitro derivatives individually at 2 or 5 µM concentrations. Among these three compounds, PLBs sections cultured on medium supplemented with nitro compound (5c) at 5 µM concentration produced maximum number (95) of plantlets. Key words: Benzimidazole derivatives, protocorm like bodies, plant growth regulators, 2-mercapto-N-methyl imidazole.
Highlights
The presence of imidazole and benzimidazole moieties in biological systems such as histidine, vitamin-B12 etc. has proven their biological relevance (Sorrell, 1989)
protocorm like bodies (PLBs) sections were cultured on half strength modified Murashige and Skoogs (MS) medium alone and modified MS medium supplemented with the synthesized chloro, methyl and nitro derivatives individually at 2 or 5 μM concentrations
In vitro production of somatic embryos/PLBs from explants of Oncidium are controlled by several factors including nature of explants, sugar source, macro and micro nutrients, vitamins, amino acids, and plant growth regulators (Chen and Chang, 2001; 2002; 2003a; 2003b; 2004; Su et al, 2006)
Summary
The presence of imidazole and benzimidazole moieties in biological systems such as histidine, vitamin-B12 etc. has proven their biological relevance (Sorrell, 1989). Reports are available where imidazole and benzimidazole derivatives have been. PLBs of orchids are the versatile system to study the effect of plant growth regulators on plant morphogenesis. In vitro production of somatic embryos/PLBs from explants of Oncidium are controlled by several factors including nature of explants, sugar source, macro and micro nutrients, vitamins, amino acids, and plant growth regulators (Chen and Chang, 2001; 2002; 2003a; 2003b; 2004; Su et al, 2006). Literature survey indicates that very little attention has been paid to the investigation of the benzimidazole derivatives (5a-c) for their effect on production of plantlets in Oncidium Gower Ramsey. 2-mercapto-N-methyl imidazole substituted benzimidazole derivatives were synthesized, characterized and studied for their effects on production of plantlets from PLBs sections of Oncidium
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