Abstract
This research focused on isolation of Silver Nano Particles (AgNPs) using leaf filtrate of Ocimum sanctum and evaluation of their antibacterial action against Bacillus aureus, Staphylococcus aureus and Escherichia coli. It is known that plant extracts act as reducing agents, stabilizers and as capping agents. The O. sanctum plant was specifically selected due to its medicinal properties and abundant availability. The change in color of leaf extract was imminent when the silver nitrate was added to the basil leaf filtrates, indicating the formation of AgNPs. The UV-visible spectroscopy and SEM analysis confirmed the formation of AgNPs. Silver ions and nanoparticles are severely toxic to microorganisms; hence they are used predominantly as antimicrobial agents. The silver nanoparticles that were synthesized from O. sanctum leaf extract have exhibited antibacterial activity against Klebsiella, B. aureus, S. aureus and E. coli and the activity varied significantly with the different concentrations of silver nitrate used. The nano particles thus developed have been confirmed by UV along with SEM reports. AgNPs sizes varied from 53.3 to 121 nm. It has been observed that most of the plant extracts yielded silver nano particles of size less than 100 nm. However, the present experimental work yielded particle size of about 121 nm from O. sanctum leaf extract. Key words: Silver nano particles (AgNPs), Ocimum sanctum, antibacterial activity.
Highlights
Nano technology is an interesting area of recent investigations with design, formation and structuring of particle in the range of 1-100 nm
Shahnaz and Mussarat (2016) conducted studies on AgNPS using Salix alba bark extract and antibacterial activity of these AgNPs was evaluated by disc diffusion, against bacteria isolated from dental plaque
The aim of the studies is to evaluate the antibacterial activity of the AgNPS against Klebsiella pneumonia, Staphylococcus aureus, Enterobacter aerogenes, Escherichia coli and Pseudomonas aeruginosa
Summary
Nano technology is an interesting area of recent investigations with design, formation and structuring of particle in the range of 1-100 nm. The luster comes along with thin layer that encompasses silver and copper NPs cover consistently in the glassy matrix of the ceramic glaze. These NPs were made by skilled people by pouring salts of Cu and Ag and oxides along with vinegar, ochre, and clay on the exterior of pottery that were sparkled previously. Azam et al (2017) studied generation of AgNPs from aqueous solution of AgNO3 by using Nigella arvensis L. seed extract and generated NPs were found to exhibit good cytotoxic effect on human breast cancer cells. The aim of the studies is to evaluate the antibacterial activity of the AgNPS against Klebsiella pneumonia, Staphylococcus aureus, Enterobacter aerogenes, Escherichia coli and Pseudomonas aeruginosa
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