Abstract
An investigation was undertaken to evaluate the effectiveness of carbon and nitrogen sources on mycelial growth and sporulation of Trichoderma harzianum (Th14). Among the tested different carbon sources viz., jaggery, honey, sugar, dextrose and peptone, significantly maximum biomass and sporulation was observed in honey (1190 mg; 7.06×108) followed by dextrose (1037 mg; 5.27×108) and jaggery (992 mg; 5.50×108) whereas among tested nitrogen sources viz., ammonium sulphate, sodium nitrate, potassium nitrate, urea, ammonium nitrate and calcium nitrate, significant maximum biomass was observed in ammonium sulphate (1035 mg) followed by sodium nitrate (965 mg), and ammonium nitrate (955 mg). However, in sporulation these nitrogen sources were at par with each other. The present result would be helpful in enhancing the conidia and biomass production of local strain and great importance when considering the production of T. harzianum for use as a biocontrol agent. Key words: Carbon sources, nitrogen sources, biomass, sporulation, Trichoderma harzianum.
Highlights
Pathogens cause world-wide economically significant diseases in numerous agricultural, horticultural and ornamental crops
The biomass and sporulation increased with increasing concentrations in amended potato broth media
Significant maximum biomass was observed at 3.0% concentration with all the carbon sources compare to 1.0 and 2.0% concentration
Summary
Pathogens cause world-wide economically significant diseases in numerous agricultural, horticultural and ornamental crops. Trichoderma strains have received particular attention as bio control agent of fungal plant pathogens. The effort for isolating and developing the indigenous bio control agents against various soil-borne plant pathogenic fungi continually conducted in several countries for alternative in reducing application of chemical pesticide (Said, 2007). Our experiment was focused on the effects of different carbon and nitrogen sources on growth and sporulation of T. harzianum in in-vitro. After 12 days incubation (DAI) the fungal mycelial mat along with spores in each flask (100 ml broth) of each treatments were separated by Whatman paper No.. The prepared powder was properly mixed in their respective flask One ml of this suspension, well shaken, was added to 9 ml of sterilized distilled water to make 10-1 dilution.
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