English

H S Fonseca Thaisa,A Vieira Filho Sidney,G N O Busatti Haendel,A Gomes Maria,P Duarte Lucienir,O Chacon Michelle,M Bertollo Caryne,R A Batista Carla,M Souza Fagundes Elaine,C Silva Fernando

doi:10.5897/ajpp2014.4129

Abstract

Species of genus Maytenus represents promising sources of bioactive compounds, mainly pentacyclic triterpenes of medicinal interest. The properties of crude extracts and three known triterpenes obtained from branches and roots of Maytenus gonoclada, against Entamoeba histolytica and human promyelocytic leukemia HL-60 (myeloid leukemia), and Jurkat (lymphocytic leukemia) cells were in vitro evaluated. For crude extracts, lupeol and 3-oxo-11α-hydroxylup-20(29)-ene activity were not observed against E. histolytica, and neither against leukemic cells. Nevertheless, in relation to tingenone considerable biological effect was detected. The cytotoxic effect of tingenone was evaluated upon human peripheral blood mononuclear cells. The cytotoxic dose showed higher efficacy, compared to the other assays. The present study signalizes the importance of tingenone, as well as its use in the production of derivatives with potential antiparasitic and antitumoral properties. Key words: Maytenus gonoclada, anti-Entamoeba histolytica activity, anti-leukemic cells activity.

Highlights

The properties of crude extracts and three known triterpenes obtained from branches and roots of Maytenus gonoclada, against Entamoeba histolytica and human promyelocytic leukemia HL-60, and Jurkat cells were in vitro evaluated
About 500 million people worldwide are annually infected with E. histolytica (Herbinger et al, 2011), which is responsible for 50 million clinical cases of dysentery or amoebic liver abscess, culminating in about 100.000 deaths (Tanyuksel and Petri, 2013)
Crude extracts from branches and roots and the triterpenes 3-hydroxy-24,29- dinor-1(10),3,5,7friedelatetraen-2,21-dione, 3oxo-11α-hydroxylup-20(29)-en and 3βhydroxylup- 20(29)-en (Figure 1), both isolated from non-polar extracts of M. gonoclada, were subjected to assays aiming to evaluate its activity against the protozoan E. histolytica

Summary

Introduction

The properties of crude extracts and three known triterpenes obtained from branches and roots of Maytenus gonoclada, against Entamoeba histolytica and human promyelocytic leukemia HL-60 (myeloid leukemia), and Jurkat (lymphocytic leukemia) cells were in vitro evaluated. Crude extracts from branches and roots and the triterpenes 3-hydroxy-24,29- dinor-1(10),3,5,7friedelatetraen-2,21-dione (tingenone) (compound 1), 3oxo-11α-hydroxylup-20(29)-en (compound 2) and 3βhydroxylup- 20(29)-en (lupeol) (compound 3) (Figure 1), both isolated from non-polar extracts of M. gonoclada, were subjected to assays aiming to evaluate its activity against the protozoan E. histolytica.

Results

Conclusion