Abstract
This study focused on the isolation and amplification of the cDNA clone of oleosin from oil palm. The oil palm cDNA library constructed from the kernel tissues 10 weeks after anthesis (WAA) produced a clone of pO1A containing partial 563 bp sequence of oleosin. The polymerase chain reaction for the rapid amplification of cDNA ends (RACE-PCR) was performed to obtain full length cDNA of oleosin from the RNA transcripts using forward gene-specific primers that is, primer Oleo 3. The product was then cloned into pCR 4 TOPO vector and subjected to sequencing using M13 forward and reverse primers. The end-to-end PCR method was performed to amplify the complete cDNA sequence of oleosin and it produce a 750 bp PCR product. The BLAST results showed that the cDNA from oil palm kernel isolated exhibited high similarities with low molecular weight isoforms of oleosin cDNA from crops like rice, maize and barley. Keywords: Oleosin, oil palm, cDNA, RACE-PCR, gene-specific primers.
Highlights
Oil palm (Elaeis guineensis Jacq.) is a perennial tree crop and has been known as the highest oil yielding crop compared to many other oil crops
This study focused on the isolation and amplification of the cDNA clone of oleosin from oil palm
The oil palm cDNA library constructed from the kernel tissues 10 weeks after anthesis (WAA) produced a clone of pO1A containing partial 563 bp sequence of oleosin
Summary
Oil palm (Elaeis guineensis Jacq.) is a perennial tree crop and has been known as the highest oil yielding crop compared to many other oil crops. Oleosins has been proposed to act as a recognition signal for the specific binding of lipase to oil bodies (Hsieh and Huang, 2004). It is necessary to understand the correlation of oleosins with the lipolytic action of lipase which decreases the quality of palm oil. Basic information regarding the molecular aspects of oil palm oleosins will be useful for verifying the proposed role of oleosins as a receptor for lipase binding (Murphy, 1993; Huang, 1996; Murphy and Vance, 1999). The objective of the study is to isolate and amplify the oleosin cDNA sequence as it is an essential step in obtaining basic information regarding the molecular aspects of oil palm oleosin and to give possible biotechnological application in other potential industries
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