Abstract
Clarified sugar cane juice was evaluated as an alternative substrate for the batch production of polyhydroxyalkanoates (PHAs) by Alcaligenes latus , and a mineral salt broth was used as the control. The study included the physicochemical characterization of the juice, measurement of the fermentation kinetic parameters and identification of the polymer type by attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy. Batch-type aerobic fermentations were performed (33°C at 200 rpm and pH 6.5-7 for 60 h) and set at 20 gL -1 of fermentable sugar and a carbon/nitrogen ratio of 28.3/1. A 10% v/v ratio of inoculum/substrate was used. The alternative substrate presented a greater concentration of magnesium and micronutrients such as Fe, B, Zn, Mg and Cu compared to the control. The biomass yield ( ) of the juice was 1.27 g.g -1 (0.414 g.g -1 in control medium), which was 69% more than the control medium; the product-substrate yield ( ) was 0.10 g.g -1 , which was similar to the control medium (0.15 g.g -1 control medium). The production of PHB was of 1.3 gL -1 , less than half of the concentration obtained in commercial substrate. Infrared spectroscopy indicated that the polymer obtained in the evaluated substrates was polyhydroxybutyrate (PHB). The clarified juice of sugar cane, without the addition of nutritional supplements can be used for the production of biomass of A. latus , first step in the production of PHB. Keywords: Alternative substrate, Alcaligenes latus, polymer, polyhydroxybutyrate (PBH), fermentation. African Journal of Biotechnology , Vol 13(40) 4019-4027
Highlights
L and d Parrado--Saboya Da arly Silvan na Faculta ad de Ingenie ería y Adminis stración, Univ versidad Nacio onal de Colom mbia Sede Pa almira, Colom mbia
Clarified su ugar cane juice was ev valuated as an alternattive substra ate for the batch produ uction of polyhydrox xyalkanoates s (PHAs) by Alcaligenes latus, and a mineral sallt broth was used as the e control
To test the hypothesis of the differences between treatments, the treatment time and treatment type according to the interaction period, structure of the compound symmetry covariance and first-order autoregressive covariance were used based on the characteristics of the repeated measurements in the experiment
Summary
L and d Parrado--Saboya Da arly Silvan na Faculta ad de Ingenie ería y Adminis stración, Univ versidad Nacio onal de Colom mbia Sede Pa almira, Colom mbia. Clarified su ugar cane juice was ev valuated as an alternattive substra ate for the batch produ uction of polyhydrox xyalkanoates s (PHAs) by Alcaligenes latus, and a mineral sallt broth was used as the e control. The study included the physicoche emical charac cterization o of the juice, m measuremen nt of the ferm mentation kinetic parrameters and identificattion of the polymer ty ype by atten nuated totall reflectance e Fourier transform infrared Batch-type ae erobic fermentations werre performed d (33°C at 200 rpm an nd pH 6.5-7 for f 60 h) and d set at 20 gL g -1 of ferme entable suga ar and a carrbon/nitrogen n ratio of. The alternativ ve substrate e presented a greater concentratiion of magne esium and micronutrient m ts such as Fe e, B, Zn, Mg and Cu com mpared to the e control
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