Abstract
Synthetic polymers are non-degradable and accumulated in the environment so, the efforts of scientists were forwarded to provide us with alternative environmentally biopolymers. Polyhydroxyalkanoates (PHAs) including polyhydroxybutyrate (PHB) are Group of the interesting biopolymers which have several medical applications such as drug delivery, suture, scaffold and heart valves. PHAs and are biological macromolecules, thermoplastics, biodegradable and biocompatible. In this study, new bacterial isolates from Egypt were screened for their ability to produce PHB using Nile red dye. Out of 44 isolates, 19 bacterial isolates were selected according to strong of their fluorescence on mineral salt medium (MSM) agar plates supplemented with Nile red. The most potent strain was identified using biochemical tests as Bacillus sp. N-2. Production of PHB was carried out in limitation of nitrogen source using minimal salt medium (MSM) supplemented with excess of glucose as sole carbon source. PHB was accumulated in relation with cell dry weight about 20% (PHB/CDW). The obtained biopolymer was purified and analyzed using NMR, FTIR, TGA and DSC thus; it was highly pure and identified as PHB. Optimization of PHB production from cheap sources appears to be a realistic goal in the future for reducing the costs and obtaining high yield.
Highlights
The great use of petroleum plastics is the major cause of environmental pollution because the plastics need years for degrading to soluble monomers and many types of toxic gases are drawback during the decomposition process (Anderson and Dawes, 1990; Suriyamongkol et al, 2007; Campos et al, 2014)
The isolated strains were grown on mineral salt medium (MSM) which was prepared according to Schlegel et al (1961) and supplemented with 20 g/l glucose as carbon source (Aramvash et al, 2015)
Different isolates obtained were streaked on plates contained MSM agar medium supplemented with glucose (2%) and Nile red dye (0.5μg/ml)
Summary
The great use of petroleum plastics is the major cause of environmental pollution because the plastics need years for degrading to soluble monomers and many types of toxic gases are drawback during the decomposition process (Anderson and Dawes, 1990; Suriyamongkol et al, 2007; Campos et al, 2014). Polyhydroxyalkanoates (PHA) which fit Hassan et al / Journal of Applied Pharmaceutical Science 6 (04); 2016: 046-051. The structure, physico-chemical properties, monomer composition, mass and size of PHAs differ considerably (Ha and Cho, 2002). The most surely understood structure of PHAs is poly-3hydroxybutyrate (PHB), a scl-PHA which can be synthesized by a wide range of microorganisms but the main interest group is Bacillus strains due to lack toxicity of the extracted PHB (Gahlawat et al, 2012; Singh et al, 2009). Amara et al (2013) reported the interests of scientists to produce biopolymers and biotechnological products with the safe method to be suitable for different applications The most surely understood structure of PHAs is poly-3hydroxybutyrate (PHB), a scl-PHA which can be synthesized by a wide range of microorganisms but the main interest group is Bacillus strains due to lack toxicity of the extracted PHB (Gahlawat et al, 2012; Singh et al, 2009). Amara et al (2013) reported the interests of scientists to produce biopolymers and biotechnological products with the safe method to be suitable for different applications
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