Abstract
The present investigation was carried out with a view to standardize an in vitro culture technique for mass propagation of an endangered medicinal plant Rauwolfia serpentinaBenth. Shoot tip was used as explant for initial culture. The explants were cultured on MS (Murashige and Skoog, 1962) medium supplemented with different concentration and combination of NAA and BA for primary shoot proliferation. The best shoot proliferation was observed in MS medium containing 0.1 mg L-1 NAA and 2.5 mgL-1 BA, where 92% of plants showed proliferation. For rooting, half strength MS medium supplemented with 0.4 mgL-1NAA and 0.1 mgL-1 IBA showed maximum root formation (91%). After acclimatization and transplantation, 90% of the in vitro derived plants survived in ex vivo condition. Key words: Rauwolfia serpentina, in vitro culture, naphthalene acetic acid, benzyl adenine, indole buteric acid, MS medium.
Highlights
Sarpagandha (Rauwolfia serpentina) belongs to family Apocynaceae
The surface sterilized shoot tips were cultured on Murashige and Skoog (MS) medium supplemented with inositol (100 mg l-1), thiamine-HCL (0.5 mg l-1), pyridoxine-HCL (1 mg l-1), nicotinic acid (0.5 mg l-1), sucrose (30 g l1) and different concentration and combination of NAA (Naphthalene acetic acid) and BA (Benzyl adenine)
The explants cultured on MS medium supplemented with different concentrations and combination of NAA and BA showed varied response for shoot formation
Summary
Sarpagandha (Rauwolfia serpentina) belongs to family Apocynaceae. In Sanskrit sarpagandha means one which smells like serpent. The plant is indigenous to India, Bangladesh and other regions of Asia and found to grow in the wild in many parts of the country (Ghani, 1998). The roots are bitter, acrid, laxative, thermogenic, diuretic and possess sedative properties. It is highly reputed for hypertension and is useful in stangury, fever, wounds, insomnia, epilepsy and dyspepsia (Prakash, 2001). 57 40 45 50 53 63 75 92 cuttings leads to destructive harvesting In view of this there is an urgent need to develop in vitro methods for the micro propagation and conservation of this valuable endangered medicinal plant. The present study was undertaken to develop more efficient protocol for in vitro propagation system using shoot tip as an explant
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