Abstract
Plants of Browallia speciosa were treated with different concentrations of sodium azide (0, 200, 400, 600 and 800 ppm) as a soil drench. The concentration of 800 ppm had simulative effect on the most studied traits. It increased the number of branches and leaves, chlorophyll content, fresh weights of vegetative growth and roots, dry weights of vegetative growth and roots and root length in the M1 (2011/ 2012) and M2 (2012/ 2013). All the concentrations of sodium azide produced changes in the flower colour, flower shape and leaf form in both generations. Peroxidase isozyme pattern indicated that seven loci produced of peroxidase are shown in B. speciosa. In addition, different peroxidase profiles among mutants were found. The phylogenetic tree indicated that mutant 4 was the most genetically distinct mutant from the control followed by mutant 3. Key words: Browallia speciosa, mutation, sodium azide, isozyme.
Highlights
IntroductionThe plants are perennials in warmer climates, native to the tropical parts of South America, but are usually grown as annuals elsewhere
Browallia is a genus of flowering plants in the Solanaceae family
The concentration of 800 ppm had simulative effect on the most studied traits. It increased the number of branches and leaves, chlorophyll content, fresh weights of vegetative growth and roots, dry weights of vegetative growth and roots and root length in the M1 (2011/ 2012) and M2 (2012/ 2013)
Summary
The plants are perennials in warmer climates, native to the tropical parts of South America, but are usually grown as annuals elsewhere. They grow well in the shade, and are frequently used as a color source in mass plantings or hanging baskets. The efficiency of mutant production depends on many conditions such as pH, soaking into water, temperature, concentration of azide and treatment duration. It creates point mutation and damages the chromosomes and produces tolerance in the plants for numerous adverse conditions (Al-Qurainy and Khan, 2009). Isozymes are widely used as molecular marker to distinguish mutants as reported by Talukdar (2010)
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