Abstract
Proteomic evidences can be pivotal to the discovery of new plant proteins and plant relationships, due to the diversity of form it can reveal. Seed storage protein profiles of 20 Fabaceae species: 4 grainlegumes and 16 non-pulses; of 16 genera and 10 tribes were analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) to estimate protein content diversity and the possible genetic relatedness. 28.3% similarity and 71.7% proteomic polymorphism was scored for the species. The high variability expressed by the lot reflects the genetic diversity amongst Fabaceae population. Dendrogram based on the proteomic data clustered the species into four groups. Aside two species, Albizia lebbeck and Albizia zygia belonging to the tribe Ingeae and those of the tribe Caesalpinieae , the other species clustered with several other non-traditional cohorts resulting in a rearrangement that showed least semblance with phylogenetic relationships based on traditional morphology taxonomic delimitation. The similarity in profiles can be preliminarily forensic for proteins of importance whether for nutritional, industrial or for improvement of existing crops or for entirely new plants as crops. The protein mix, and the resultant relationship based on seed storage proteins instigates a review of erstwhile taxonomic, agricultural and research perspectives for the Fabaceae. Keywords : Fabaceae, seed protein, polymorphism, genetic diversity, taxonomy, single protein African Journal of Biotechnology Vol. 12(17), pp. 2157-2163
Highlights
Legumes vary from annual and perennial herbs to shrubs, trees, vines/lianas, and even a few aquatics; in size from some of the smallest plants of deserts and arctic or alpine regions to the tallest of rain forest trees as well as constitute conspicuous, and often dominant component of most of the vegetation types distributed throughout temperate and tropical regions of the world (Rundel, 1989)
Seed storage protein profiles of 20 Fabaceae species: 4 grainlegumes and 16 non-pulses; of 16 genera and 10 tribes were analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) to estimate protein content diversity and the possible genetic relatedness. 28.3% similarity and 71.7% proteomic polymorphism was scored for the species
The SDS gel electrophoresis of reproducible storage proteins for the 20 Fabaceae species resulted in bands that ranged from molecular weights of 14 to >100 kDa
Summary
Legumes vary from annual and perennial herbs to shrubs, trees, vines/lianas, and even a few aquatics; in size from some of the smallest plants of deserts and arctic or alpine regions to the tallest of rain forest trees as well as constitute conspicuous, and often dominant component of most of the vegetation types distributed throughout temperate and tropical regions of the world (Rundel, 1989). Legumes are diverse in tropical forests and temperate shrub lands with a seasonally dry or arid climate. This preference for semiarid to arid habitats is related to a nitrogen-demanding metabolism (Sprent and McKey, 1994; Sprent, 2001). The family Fabaceae is traditionally divided into three subfamilies, the Caesalpinioideae, Mimosoideae and Papilionoideae; a recognition that is based mainly on floral characteristics with 39 tribes and some 670 genera recognized (Polhill and Raven, 1981; Polhill, 1994). Recent update of the tribal and generic re-evaluation of the classification of Fabaceae, have resulted from more than 10 years of intensive molecular phylogenetic studies; recognizes 36 tribes, 727 genera and 19,327 species (Lewis et al, 2005)
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