Abstract
The most economically damaging ilarvirus affecting hazelnut on a worldwide scale is the related apple mosaic virus (ApMV). Attempts were made to isolate the virus RNA from hazelnut tissues using different extraction methods. The most suitable extraction method that could detect the virus occurring naturally in hazelnut by reverse-transcription polymerase chain reaction (RT-PCR) methodology was selected. RT-PCR was applied successfully using flower, husk and leaf tissues. The most suitable extraction method and hazelnut tissues determined were sensitive, simple, rapid and reliable for simultaneous detection of ApMV in hazelnut tissues. To our knowledge, this is the first report of the simultaneous detection of the virus by RT-PCR, an alternative detection of ApMV in hazelnut hosts. Key words: RT-PCR, hazelnut, apple mosaic virus.
Highlights
Apple mosaic virus (ApMV), a member of the genus Ilarvirus (Fulton, 1972), occurs worldwide and infects a number of woody plants of over 65 species in 19 families including rose, hop, birch and raspberry (Brunt et al, 1996; Gotlieb and Berbee, 1973; Postman and Cameron, 1987; Sweet and Barbara, 1979; Wong and Horst, 1993) and induces bright yellow patterns on leaves (Nemeth, 1986)
ribonucleic acid (RNA) prepared with these buffer systems and active charcoal served as a robust template for reverse transcription as indicated by Polymerase chain reaction (PCR) amplification from cDNA for hazelnut isolates of apple mosaic virus (ApMV)
The fast and simple alternative detection method with one tube reverse-transcription polymerase chain reaction (RT-PCR) can help minimize the time and labour required for the diagnosis of ApMV in hazelnut
Summary
Apple mosaic virus (ApMV), a member of the genus Ilarvirus (Fulton, 1972), occurs worldwide and infects a number of woody plants of over 65 species in 19 families including rose, hop, birch and raspberry (Brunt et al, 1996; Gotlieb and Berbee, 1973; Postman and Cameron, 1987; Sweet and Barbara, 1979; Wong and Horst, 1993) and induces bright yellow patterns on leaves (Nemeth, 1986). Serological detection by ELISA using commercially available antisera represents the first alternative for biological indexing, but its use is limited to certain time period in the growing season and appears inappropriate with dormant woody tissues (Postman and Cameron, 1987; Aramburu and Rovira, 2000; Kobylko et al, 2005).
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