Abstract
Eighty-five uropathogen isolates were collected and differentiated on the basis of their Gram stain reaction. Among the collected isolates, 25 (29.4%) were Gram positive cocci and 60 (70.6%) were Gram negative bacilli. Antibiotic susceptibility profile towards 15 different antibiotics concluded that impenime (IMP), amikacin (AK) and Pipperacillin/tazobactam (TZP) were the most effective against Gram negative; while linezolid (LZD), and vancomycin (VA) were the most potent against Gram positive. The highly drug resistant isolates were identified as Pseudomonas aeruginosa and Klebsiella pneumoniae using 16srRNA; the two strains were subjected to different doses of gamma-radiation and the sub lethal dose of both strains was 5.0 kGy. The D10 values were recorded (1.2 and 1.1 kGy) for P. aeruginosa and K. pneumoniae), respectively. In addition, gamma-irradiation technique decreases the resistance of K. pneumoniae towards IMP and the resistance of P. aeruginosa towards more than one antibiotic (TZP, AZM and CIP). The results also, revealed that both of the tested strains had the ability to produce Extended Spectrum β-lactamase (ESBL) before and after gamma-radiation but only P. aeruginosa had the ability to produce Metallo β-lactamase (MBL), that is, ESBL and MBL co-production was detected in P. aeruginosa. So, these findings must be supported by other studies on the level of genes to prove the possibility for using gamma-irradiation technique to overcome microbial drug resistance problem. Key words: Urinary tract infection, antibiotic resistance, β-lactamases, gamma radiation.
Highlights
Urinary tract infection (UTI) is one of the most common human bacterial infections both in the community and hospital setting and accounts for one-third of all nosocomial infections (Dougnon et al, 2020)
Tryptone Glucose Yeast Extract Agar (TGY), Nutrient agar (NA), Blood agar (BA) and MacConkey agar were purchased from Difco [Difco Labs, Detroit, Michigan (USA)]
The results revealed that among all the examined isolates, 25 isolates (29.4%) were Gram positive cocci and 60 (70.6%) were Gram negative bacilli or short rods
Summary
Urinary tract infection (UTI) is one of the most common human bacterial infections both in the community and hospital setting (the health care settings) and accounts for one-third of all nosocomial infections (Dougnon et al, 2020). Infection of urinary tract marked a range of severity that spans from mild self-limiting infection to life threatening systemic disease (Lee and Kim, 2015; Klein and Hultgren, 2020). UTI causes many urinary disorders as urosepsis, renal scarring and progressive kidney damage that lead to a high health risk with high mortality, morbidity and cost a significant financial burden (Gashti et al, 2020).
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