Abstract
The genetic diversity of bovine viral diarrhea viruses (BVDV) from Beijing region, China between 2009-2010, was investigated using a phylogenetic analysis of partial 5’untranslated region (5’UTR) nucleotide sequences from 61 BVDV positive samples, and for 13 selected samples, the Npro region was also investigated. All the sixty-one blood samples were collected from cattle that were BVDV persistently infected (PI) within a BVD eradication program on Beijing farms during 2009 and 2010. Phylogenetic analysis based on 5’UTR sequences indicated that all BVDV positive samples were of the BVDV-1 genotype, but were clustered into four different subtypes: sub-genotype 1b (n = 31), 1m (n = 24), 1c (n = 4) and 1d (n = 2). BVDV-1b and 1 m are the most prevalent sub-genotypes in Beijing. The results also demonstrate that in most herds, certain BVDV-1 sub-genotype predominates. It is the first time that BVDV-1d is reported in China with unclear origin. The result of this study will be useful in constructing an effective vaccination plan to further control BVDV in Beijing region. Key words: bovine viral diarrhea viruses 1 (BVDV-1), 5’untranslated region (5’UTR), Npro, phylogenetic analysis.
Highlights
Bovine viral diarrhea virus (BVDV), a member of the genus Pestivirus, family Flaviviridae, causes significant losses in cattle farming worldwide (Vilcek et al, 2001)
After screening persistently infected cows in Beijing dairy herds, we investigated the genetic diversity of bovine viral diarrhea viruses (BVDV) obtained in positive serum samples by performing phylogenetic analysis of partial 5’untranslated region (5’UTR) genome part, and for selected samples the Npro region to check the results was obtained
The genetic diversity of BVDV field positive samples was surveyed by determining the nucleotide sequences of the 5’UTR of 61 positive samples and the Npro region of 13 selected samples, obtained in Beijing between 2009 and 2010
Summary
Genetic diversity of bovine viral diarrhoea virus in Beijing region, China from 2009 to 2010. The genetic diversity of bovine viral diarrhea viruses (BVDV) from Beijing region, China between 2009-2010, was investigated using a phylogenetic analysis of partial 5’untranslated region (5’UTR) nucleotide sequences from 61 BVDV positive samples, and for 13 selected samples, the Npro region was investigated. Phylogenetic analysis based on 5’UTR sequences indicated that all BVDV positive samples were of the BVDV-1 genotype, but were clustered into four different subtypes: sub-genotype 1b (n = 31), 1m (n = 24), 1c (n = 4) and 1d (n = 2). BVDV-1b and 1 m are the most prevalent sub-genotypes in Beijing. The results demonstrate that in most herds, certain BVDV-1 sub-genotype predominates.
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