Abstract
The effects of wall materials and encapsulation by lyophilization on the viability of Weissella confusa were evaluated. Aloe vera gel, sodium casein at 5 and 15% p/v, sodium alginate at 2% p/v, buffer phosphate, and a mixture (Aloe vera gel, sodium casein, and sodium alginate) as wall materials, were used. Bacteria without encapsulation ( W. confusa ) as control were used. Encapsulated bacteria were freeze dried for 48 h, in order to determine their viability in the freezing and sublimation-drying stages. Results indicate that bacteria without encapsulation, showed greater loss of viability in the sublimation-drying stage. All the wall materials evaluated, may be used for encapsulation of bacteria, because, at the end of the freeze-drying process, the encapsulated bacteria showed higher viability percentages than non-encapsulated bacteria, with significant statistical difference (p<0.05). The protective effect of wall materials was higher in the sublimation-drying stage, compared to freezing stage. Keywords: Aloe, Weissella , probiotic, encapsulation. African Journal of Biotechbiology , Vol 13(26) 2661-2667
Highlights
Probiotic lactic acid bacteria (LAB) are useful for the dairy and nutraceutical industry, due to their applications to human and animal health (Reddy et al, 2009)
The life of the bacteria was protected in higher percentage in the sublimation-drying stage
All wall materials evaluated in this study, have potential in the encapsulation of lactic acid bacteria, due to, cell counts at the end of the process, are found within the ranges accepted by several conuntries, for probiotics foods
Summary
Probiotic lactic acid bacteria (LAB) are useful for the dairy and nutraceutical industry, due to their applications to human and animal health (Reddy et al, 2009). In the food probiotics, processing is important; the concentration of probiotic bacteria (WHO / FAO, 2006), and the techniques are used to maintain their viability (Carvalho et al, 2004). Encapsulation techniques have been developed and successfully used in the preservation and protection of probiotic LAB. The material used to trap the substance or microorganism to be encapsulated is called encapsulation material, cover membrane, shell, vehicle, wall material, or external phase matrix (SernaCock and Vallejo-Castillo, 2013). Encapsulation of LAB reduces damage caused by external factors such as storage conditions (time, temperature, moisture, oxygen)
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