Optimization of Aloe vera and alginate as encapsulating matrices for Lactobacillus acidophilus using FCCD-RSM approach

Abstract

In order to maintain the viability of probiotic, the used of prebiotic is important. Aloe vera has been hypothesized to be a prebiotic potential for probiotic. Due to low viability of encapsulated cells, Aloe vera gel was used to be part of enacapsulating matrices for probioctic, Lactobacillus acidophilus. As a result, optimization of Aloe vera gel with sodium alginate was carried out in order to determine the maximum encapsulation yield. Face centered composite design-response surface methodology (FCCD-RSM) was employed to optimize the sodium alginate concentration and Aloe vera gel (prebiotic) composition during microencapsulation process in performing a better encapsulation yield for L. acidophilus. The encapsulation yield (EY) of encapsulated L. acidophilus was investigated with respect to two variables which were sodium alginate (X <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1</inf> ) and Aloe vera gel (X <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</inf> ). Result obtained with polynomial regression model used in this study showed highly significant (0.0013) with R <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> value was 0.9138. Aloe vera gel was showed highly significant (p<0.05) effect to the encapsulation yield. The optimum Aloe vera gel and sodium alginate were obtained at 1.12% (v/v) and 1.28% (w/v), respectively. Based on the verification process, the experimental and predicted results were showed not significant difference (p< 0.05).