Abstract
The aim of this work was to evaluate the effect of sucrose to reduce the in vitro growth of cassava plants using multivariate statistical tests. Cassava conservation has a relevant role as an auxiliary strategy for preservation and genetic breeding. Micro-cuttings of cassava accessions BGM 264, BGM 265, BGM 1037 and BGM 1282 from the Active Germplasm Bank of the Embrapa Cassava and Fruits were tested with five different concentrations of sucrose (0, 14.6, 29.2, 43.8, 58.5 mM) and the following variables were evaluated: plant height (cm), total number of leaves, number of senescent leaves, number of micro-cuttings, size of callus, number of roots and plant vigor. The data were submitted to analysis of variance (ANOVA) and multivariate analysis of variance (MANOVA). According to the criterion of Singh, the variable that contributed most to explain the behavior of cassava plants under the conditions studied was the number of leaves, with 36.52%, while the vigor of the plants showed the lowest contribution (0.66 %). The best concentration for in vitro cassava conservation was 58.45 mM of sucrose, based on the number of viable plants after incubation. Key words: Biotechnology, germplasm conservation, tissue culture, Manihot esculenta , genetic resources
Highlights
IntroductionThe genetic diversity of cassava in Brazil provides a wide genetic base for breeding programs in the tropics
The genetic diversity of cassava in Brazil provides a wide genetic base for breeding programs in the tropics. This variability covers a group of genes that confer resistance to major pests and diseases that affect the crop and enables adaptation to different edaphoclimatic conditions (Albuquerque et al, 2009)
The aim of this work was to evaluate the effect of sucrose in reducing the in vitro growth of cassava plants using multivariate statistical tests, aimed at more efficient study of in vitro conservation of cassava germplasm
Summary
The genetic diversity of cassava in Brazil provides a wide genetic base for breeding programs in the tropics. This variability covers a group of genes that confer resistance to major pests and diseases that affect the crop and enables adaptation to different edaphoclimatic conditions (Albuquerque et al, 2009). Genetic erosion in cassava is mainly caused by biotic and abiotic stresses, which along with the expansion into new agricultural frontiers is a fact that should not be ignored in genetic breeding program, to avoid jeopardizing present and future actions.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
