Abstract
Samples of 320 random healthy unrelated Iraqi male persons from the Arab ethic group of Iraqi population were collected for Y chromosomal STRs typing. FTA® Technology was utilized to extract DNA from blood collected on FTA™ paper. We analyzed 11 Y chromosomal STR loci for evaluating allele frequencies and gene diversity for each Y-STR locus of the Y-Plex™ PCR amplification kit. The 11 loci include DYS389I, DYS389II, DYS19, DYS391, DYS438, DYS390, DYS439, DYS392, DYS393, DYS385a and DYS385b. A total of 299 unique haplotypes was identified among the 320 individuals studied. The DYS385b had the highest diversity (GD = 0.8392), while loci DYS392 had the lowest (D = 0.2695). The study focuses to establish the basic forensic genetic informations, knowledge, data and statistics which might be so ultimately helpful practically in forensic science and criminology and to let evaluate and present the DNA weight evidences in Iraq medico-legal institute and courts of law. Key words: Allele frequency, gene diversity, Iraq, STR DNA typing, Y-Plex™.
Highlights
Samples of 320 rando om healthy unrelated Irraqi male pe ersons from m the Arab ethic group p of Iraqi population were collec cted for Y ch hromosomal STRs typin ng
Blood samples were randomly collected from 320 unrelated Iraqi male persons from the Arab ethnic group of Iraqi population for DNA Y- chromosomal STRs typing; those samples were sent to the forensic genetic laboratories
The frequencies of DYS392 w were low in all allele e except allele e 11 where itt was 85%; th his allele is to o be ussed to discrim minate Iraqi m men because of overall low w genettic diversity, this lo ocus should be excluded d becau use 85% of Irraqi men will have the locu us
Summary
Samples of 320 rando om healthy unrelated Irraqi male pe ersons from m the Arab ethic group p of Iraqi population were collec cted for Y ch hromosomal STRs typin ng. To determine the frequency of a particular Y-STR profile, the profile must be searched against different databases for a possible match, and these databases must be large enough to accurately represent the frequencies of the haplotypes present in the population of interest (Ballantyne et al, 2010). A forensic Y chromosome haplotype should include as many polymorphic loci as possible to improve the chance of excluding individuals (or male lineages) who did not commit the crime.
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