Abstract
An attempt was made to study the somatic embryogenesis and plant regeneration from the in vitro leaf explants of Rumex vesicarius L. a renowned medicinal plant, which belongs to polygonaceae family. Effective in vitro regeneration of R. vesicarius was achieved via young leaf derived somatic embryo cultures. Embryogenic callus was induced from leaf explants on Schenk and Hildebrandt (SH) medium supplemented with various concentrations of 2,4-dichlorophenoxy acetic acid (2,4-D) (0.5 to 3.0 mg/l) along with Kinetin (Kn) (0.5 mg/l). High frequency of somatic embryogenesis was effective on SH medium with 2, 4-D (2.5 mg/l) + Kn (0.5 mg/l) from leaf explants. Secondary somatic embryogenesis was also observed when primary somatic embryos were subculture on the same somatic embryo induction medium. Well developed cotyledonary shaped embryos regenerate 80% of shoots on media containing 2,4-D 0.5 mg/l + 2.0 mg/l BA. The regenerated shoots transferred to rooting media containing Indole- 3- butyric acid (IBA). Efficient rooting of 90% was noted on SH media with 1.0 mg/l IBA. Finally, these in vitro regenerated plantlets were hardened, acclimatized and successfully transferred to the field. The post transplantation survival rate of these regenerated plants was 65 to 70%. The in vitro regenerated plants and flowers were similar to mother plants. This protocol will be useful for genetic transformation experiments in R. vesicarius L. Keywords: Rumex vesicarius L, 2,4-dichlorophenoxy acetic acid (2,4-D), kinetin (Kn), Benzyl adenine (BA), Indole- 3- butyric acid (IBA). African Journal of Biotechnology , Vol 13(45) 4268-4274
Highlights
Lavanya a Kakarla, Chakravarrthy Rama*, Mahendran Botlag gunta, Kris shna M
We found that compared to ex vitro, the in vitro leaf explants was found to be appropriate as it was responding well under in vitro conditions
The in vitro leaf explants were spliced at the terminal ends using scalpels and inoculated on SH medium containing different concentrations of 2,4-D (0.5 to 3.0 mg/l) in combination with Kn (0.5 mg/l)
Summary
Lavanya a Kakarla, Chakravarrthy Rama*, Mahendran Botlag gunta, Kris shna M. 13 October, 2014 t study the somatic embryogenesis s and plant rregeneration from the in vitro leaf An attempt was made to sicarius L. a renowned medicinal m pllant, which b belongs to p polygonacea ae family. R vesicarius s was achiev ved via you ung leaf deriived somatic c embryo cultures. Em mbryogenic callus was induced i from m leaf explan nts on Schenk and Hilde ebrandt (SH)) medium supplementted with varrious concen ntrations of 2,4-dichloro ophenoxy acetic acid (2,4-D) (0.5 to 3.0 mg/l) along with Kinetin (Kn n) (0.5 mg/l)). High frequ uency of so omatic embrryogenesis w was effectiv ve on SH medium witth 2, 4-D Second dary somatic c embryogen nesis was observ ved when prrimary somattic embryos were subcu ulture on the same soma atic embryo iinduction medium. Effic cient rooting g of 90% was s noted on S
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
