Abstract
Several reports have associated the variability in physico-chemical properties of avidin protein to dynamism inherent in the consensus regulatory networks within the promoter region of avidin genes. An Avr4 promoter region ligated to chloramphenicol acetyltransferase plasmid vector (pBLCAT2) to produce recombinant plasmid Avr4pBLCAT2 was sequentially deleted to produce five distinct mutants: Avr4pBLCAT2907-176, Avr4pBLCAT2809-176, Avr4pBLCAT2789-176,Avr4pBLCAT2429-176 and Avr4pBLCAT2302-176. The transformants elicited different chloramphenicol acetyl transferase (CAT) activities. Key words: Avidin, AVR4 promoter, chloramphenicol acetyl transferase.  
Highlights
Avidin gene when expressed in oviductal tissues of oviparous animals and in response to inflammation results in the production of a tetrameric protein (Green, 1975; Zerega et al, 2001)
These variations seem to provide indications that avidin gene display diversity in structure even within the 5’ flanking promoter region containing a network of regulatory cis elements such as hormone response elements (HRE), heat shock response element (HSE) and acute phase protein (APRE) (Yamamoto, 1985; Bienz and Phelam, 1986)
Chloramphenicol acetyltransferase plasmid vector and AVR4 promoter region ligated to pBLCAT2 to produce a recombinant plasmid Avr4pBLCAT2 was obtained from the Dept of Molecular Medicine University of Jyvaskyla, Finland
Summary
Several reports have associated the variability in physico-chemical properties of avidin protein to dynamism inherent in the consensus regulatory networks within the promoter region of avidin genes. An Avr promoter region ligated to chloramphenicol acetyltransferase plasmid vector (pBLCAT2) to produce recombinant plasmid Avr4pBLCAT2 was sequentially deleted to produce five distinct mutants: Avr4pBLCAT2907-176, Avr4pBLCAT2809-176, Avr4pBLCAT2789-176, Avr4pBLCAT2429-176 and Avr4pBLCAT2302-176. The transformants elicited different chloramphenicol acetyl transferase (CAT) activities
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