Abstract
The micropropagation of Guadua angustifolia Kunth, commonly known as giant bamboo, using semi-solid culture medium, is reported to have low multiplication rates. This study evaluated the multiplication index of G. angustifolia in a temporary immersion system (RITA®), comparing results with those obtained using a semi-solid culture medium. The treatments consisted of either three or four 2-min immersions per day and use of semi-solid culture medium, which consisted of MS supplemented with 3.0 mg L-1 of the cytokinin benzylaminopurine (BAP). Equipment consisted of 20 vessels for automated RITA®, each containing 200 ml of culture medium. Immersions were performed for 2 min at two different frequency intervals (6 and 8 h). Large clumps of G. angustifolia with 1, 2 or 3 stems were inoculated depending on the treatment. Best results were obtained with four immersion cycles per day (every 6 h), with a multiplication index of 2.7 shoots per original explant (axillary buds) and greater rhizome growth. Overall, the temporary immersion system performed better than the semi-solid medium in terms of shoot multiplication rates and rhizome growth. Further studies should be conducted to develop an application for RITA® for use in the commercial production of G. angustifolia. Key words: Giant bamboo, temporary immersion system RITA, rhizome.
Highlights
Guadua angustifolia, known as giant bamboo, belongs to the Poaceae family, one of the four largest families of the plant kingdom, harboring from 600 to 700 genera and nearly 10,000 species (Soderstrom et al, 1988)
This study evaluated the multiplication index of G. angustifolia in a temporary immersion system (RITA®), comparing results with those obtained using a semi-solid culture medium
Further studies should be conducted to develop an application for RITA® for use in the commercial production of G. angustifolia
Summary
Known as giant bamboo, belongs to the Poaceae family, one of the four largest families of the plant kingdom, harboring from 600 to 700 genera and nearly 10,000 species (Soderstrom et al, 1988). Physical and mechanical characteristics, G. angustifolia has played an important environmental, cultural and economic role in Colombia. For these same reasons, it has suffered indiscriminate exploitation. The deterioration of stands of G. angustifolia during vegetative multiplication is further justification for exploring micropropagation approaches to develop efficient mass multiplication methods for this important species using explants (Cruz, 2009)
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