Abstract
Polyhydroxyalkanoates (PHA) are biodegradable polyesters accumulated intracellularly as energy resources by bacterial species. In this study, fermentation process for production of PHA is carried out using sesame oil as carbon source. We studied recovery of poly(3-hydroxybutyric acid) (PHB) from Staphylococcus epidermidis by sodium hypochlorite digestion method. Recovered PHB sample was estimated by UV spectrophotometer. PHB from S. epidermidis was characterized and by these findings, we examined purified PHB by differential scanning calorimeter (DSC), a thermo gravimetric analyzer (TGA), thin layer chromatography (TLC) and infrared spectroscopy (IR). The results of our analysis of PHB while comparing with commercial source suggest that in DSC melting temperature of PHB was 173.36°C, TGA thermo grams of PHB sample was at 296.91°C, on TLC plate; Rf value was calculated as 0.71 and finally IR spectrum of the compounds showed characteristics bands for the groups CH, C=O and C-O, indicating the presence of PHB in the production medium. Key words: Polyhydroxyalkanoates (PHA), poly(3-hydroxybutyric acid) (PHB), Staphylococcus epidermidis.
Highlights
Often, research and media attention on the renewable bioproduct industry is focused on fuel alternatives
Research and media attention on the renewable bioproduct industry is focused on fuel alternatives. This is logical since diminishing fossil fuels supply nearly 80% of the global energy demands, and it is predicted that the current demand will increase by 56% by 2040 (U.S Energy Information Administration, 2013)
200 ml of sterile production medium, and all the isolates were first grown for 72 h. at 37°C with shaking at 150 rpm in a carbon - rich MSB medium containing sesame oil (1% w/v) as a sole carbon source and cells accumulating poly(3-hydroxybutyric acid) (PHB) were cultivated in 250 ml of modified mineral salts basal medium (MMSB) as described by Marjadi and Dharaiya (2012)
Summary
Research and media attention on the renewable bioproduct industry is focused on fuel alternatives. This is logical since diminishing fossil fuels supply nearly 80% of the global energy demands, and it is predicted that the current demand will increase by 56% by 2040 (U.S Energy Information Administration, 2013). By 2015, the demand for biodegradable plastics is estimated to reach 1.1 million tons (Metabolix, 2013a). Poly(3hydroxyalkanoic acid) (PHA) is a biodegradable polymer material that accumulates in numerous microorganisms under unbalanced growth conditions (Ribera et al, 2001). Β-hydroxybutyrate (PHB) is biopolymer that can be used as biodegradable plastics is the most common natural microbial PHA (Singh and Parmar, 2013). In terms of molecular weight, brittleness, stiffness, melting point and glass transition temperature, the PHB
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