Abstract
Lumnitzera littorea (Jack) Voigt. is a species of mangrove plant belonging to the family Combretaceae. Natural reproduction of L. littorea is extremely difficult due to its seed abortion in China. To reveal the molecular mechanism of seed abortion, we performed transcriptome to analyze the seeds of L. littorea-T (normal seeds) and L. littorea-S (abortive seeds). After analysis of the raw data, 64,868 transcripts (mean length = 658 bp) were assembled. Among these transcripts, 39,779 were functionally annotated. Then, differentially expressed genes (DEGs) were screened, and 23,513 transcripts were identified that were likely involved in seed abortion. About 207 DEGs assigned to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, and approximately 12.1% fell into reproduction categories. Genes involved in response to sucrose and starch metabolism, and phytohormone biosynthesis showed significant different expression levels between normal and abortive seeds of L. littorea. Further expressions patterns of key genes contribute to seed development were analyzed using quantitative real-time PCR, and the results were consistent with high-throughput sequencing data. The information obtained in this study will aid in the research of the mechanism of seed abortion in further molecular studies of L. littorea. © 2021 Friends Science Publishers
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
