Abstract
The current study was aimed to investigate the incidence of different Salmonellaserovars in chicken products either from local or imported source. A total of 152 samples of chicken and chicken products were collected from different retail establishment markets in Riyadh, KSA including 38 local whole frozen chickens, 62 imported whole frozen chickens, 22 whole poultry eggs and 30 local chicken cuts samples and examined by standard microbiological techniques (SMT). Salmonellaisolation revealed a total percentage of 5.92%; chicken cuts revealed a high incidence among the examined samples (10%), followed by local frozen chickens and imported frozen chicken samples with incidence of 7.89 and 4.83%, respectively. For this experiment, the whole chicken eggs were negative forSalmonella species by SMT. Salmonella enteritidis was dominating among the recovered Salmonella serovars, followed by Salmonella typhimurium, while only two strains of Salmonella agona and Salmonella newport were isolated. The PCR assay combined with Rappaport- Vassiliadis (RV) selective broth (PCR-RV) for the detection of Salmonella species in the collected field samples revealed the same positive samples directly from the imported frozen chickens and whole chicken eggs which gave negative results by SMT. Thus PCR-RV technique is rapid, time saving and applicable to detect Salmonella serovars directly from chicken samples. Key words: Frozen chickens, Salmonella serovars, diagnosis, enrichment, selective, polymerase chain reaction.
Highlights
Salmonella species live in the intestinal tracts of warm and cold blooded animals
Salmonella isolation revealed a total percentage of 5.92%; chicken cuts revealed a high incidence among the examined samples (10%), followed by local frozen chickens and imported frozen chicken samples with incidence of 7.89 and 4.83%, respectively
The standard microbiological techniques for detection of different Salmonella serovars conducted according to ISO 6579 (2002); 25 g of poultry composite samples were homogenized in a stomacher (Bag Mixer 400, Interscience, France) for 1 to 2 min in 225 ml of buffered peptone water (BPW) and incubated under aerobic conditions at 37°C for 16 - 20 h followed by selective enrichment of 0.1 in 10 ml of Rappaport –Vassiliadis (RV) broth
Summary
Salmonella species live in the intestinal tracts of warm and cold blooded animals. Some species are ubiquitous;. The isolation and identification of salmonellae from clinical samples by traditional cultural techniques requires laborious procedures which can last up to 7 days (Stone et al, 1994), so there is a need for the development of innovative methods for the rapid identification of Salmonella food-borne pathogen to over come these drawbacks. Molecular techniques such as Polymerase Chain Reaction (PCR) especially by using selective broth culture have been invaluable tools for the detection of different Salmonella species (Oliveira et al, 2003). Investigation of S. enteritidis and Salmonella typhimurium among Salmonella enterica serovars in chickens and chicken products collected from Riyadh, King Saudi Arabia (KSA) using conventional and molecular techniques (PCR using selective broth culture) was the major strategy of this study
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