Abstract
Hevea brasiliensis that is grown in Thailand and Southeast Asia is a very valuable source of natural rubber. However, few studies have been conducted to evaluate the effects of seasonal effect on plantlet regenera-tion through somatic embryogenesis from anther culture. Thus, this study discussed the effect of season on callus induction subsequent to plantlet regeneration from culturing of anther. Male flowers were aseptically opened and anthers were excised to culture on callus induction medium (CIM) which was MS supplemented with 5% sucrose, 1 mg.L-1 2,4-D, 1 mg.L-1 KN, 1 mg.L-1 NAA. Somatic embryo (SE) developed on MS medium supplemented with 3% sucrose, 0.2 mg.L-1 NAA, 1 mg.L-1 BA, 3 mg.L-1 KN and 0.05 mg.L-1 GA3. This medium is so called embryo induction medium (EIM).Anther collectedfrom different season gave different result in callus induction, proliferation and somatic embryogenesis, healthy anther collected in February 15, 2013 gave the best result in callus induction (86.25%), proliferation (422 mgFW) and somatic embryogenesis (20%). Complete plantlets (15.63%)were obtained on MS medium supplemented with 5% sucrose, 0.5 mg.L-1 KN, 0.2 mg.L-1 IAA, 1 mg.L-1 BA, 0.3 mg.L-1 GA3 and 0.2% phytagel. Seasonal collection plays influence rolein successful somatic embryogenesis in tissue culture of rubber tree. Key words: Hevea brasiliensis, rubber, antherculture, callus induction, seasonal collection, somatic embryogenesis.
Highlights
Effe ect of season s nal colllection n on ca allus In nductio on, p prolife eration and somatic s c embrryogen nesis ffrom anther
Male flowers were aseptically opene ed and anthe ers were exc cised to cultu ure on callus induction medium (CIM) which was MS sup pplemented with 5% suc crose, 1 mg.L
Inflorescen nces collected d in some sea asonsrespond ded at low frequency in callus induction ra ate (Figure 1)
Summary
Effe ect of season s nal colllection n on ca allus In nductio on, p prolife eration and somatic s c embrryogen nesis ffrom anther. Few studies s have been condu ucted to eval uate the effe ects of seaso onal effect on n plantlet regenera-tio on through somatic s emb bryogenesis from f anther culture. This study y discussed tthe effect of season on o callus induction subsequent to pla antlet regene eration from m culturing off anther. This medium is so called embry yo induction n medium (EIM).Anther co ollectedfrom m different season gav ve different result in ca allus inducttion, proliferration and s somatic emb bryogenesis, healthy anther colle ected in February 15, 201. Seasonal collection plays s influence ro olein succes ssful somatic c embryogen nesis in tissu ue culture of rubber trree
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
