Abstract
An efficient protocol for in vitro propagation of Digitalis trojana Ivan. was developed via adventitious shoot regeneration. Leaf explants were cultured on MS which were supplemented with different concentrations of NAA (0.1, 0.5, 1.0 mg/ml) and BAP (0.1, 0.5, 1.0, 3.0, 5.0 mg/ml) for shoot formation. Adventitious shoots were formed on leaf explants within three weeks in culture. The best shoot proliferation was observed among explants cultured on MS medium with 0.1 mg/ml NAA + 3.0 mg/ml BAP. Regenerated shoots were multiplicated by subculture. Then they were cultured on MS with 0.1% (w/v) activated charcoal for root formation. All of the in vitro regenerated plantlets were successfully acclimatized ex vitro and then grown healthy. Key words: Digitalis trojana Ivan., regeneration, in vitro, naphthalene acetic acid, 6-benzylaminopurine.
Highlights
In vitro culture technologies have been increasingly used for ex situ conservation of rare or endangered endemic plants (Sasaran et al, 2006; George et al, 2007)
Leaf explants were excised from twelve weeks old seedlings and cultured on murashige and skoog medium (MS) containing different concentrations of - naphthalene acetic acid (NAA) (0.1, 0.5, 1.0 mg/ml) and BAP (0.1, 0.5, 1.0, 3.0, 5.0 mg/ml) for direct shoot regeneration
The explants cultured on MS medium without hormones were only slightly expanded and no calli and shoots was observed on this medium
Summary
In vitro culture technologies have been increasingly used for ex situ conservation of rare or endangered endemic plants (Sasaran et al, 2006; George et al, 2007). It was reported that D. trojana contained the highest amount of cardiac glycosides in leaves among the endemic Digitalis species (Tanker et al, 1988). There are lots of reports about in vitro cultures of other Digitalis species (Erdei et al, 1981; Perez-Bermudez et al, 1984; Matsimoto et al, 1987; Herrera et al, 1990; Vela et al, 1991; Sale et al, 2002) but though it is a valuable medicinal and endemic plant, there is no report on an in vitro culture protocol for D. trojana. An efficient in vitro plant regeneration of D. trojana Ivan.
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