Abstract
RAPD technique was used in this study to detect DNA band variations between both normal and abnormal male of broiler chicken based on RAPD marker. DNA polymorphisms between normal and mutant birds were detected using fifteen oligonucleiotide primers. Using these primers, DNA band loss ranged from 25 to 75%. Data demonstrated that RAPD marker could detect DNA alterations.
Highlights
DNA alterations include DNA adducts formation, breaks, point mutations, large rearrangements, and other changes such as structural distortion induced by chemical or physical agents following direct and/or indirect interaction with the genomic DNA
random amplified polymorphic DNA (RAPD) profiles deriving from Escherichia coli wild and mutant strains, were compared under the hypothesis that stochastic mutations can accumulate at higher rate in the defective strains during cell replication
The RAPD assay was performed on a given DNA template with a set of primers differing by a single base in comparison to the original primer
Summary
DNA alterations include DNA adducts formation, breaks, point mutations, large rearrangements, and other changes such as structural distortion induced by chemical or physical agents following direct and/or indirect interaction with the genomic DNA. Such alteration can be detected by the random amplified polymorphic DNA (RAPD). Becerril et al (1999) have studied the capability of the randomly amplified polymorphic DNA technique to show genotoxic effects induced by chemicals in fish cells. The indicated the effectiveness of RAPD in detecting polymorphism in chicken populations, application in population studies, and establishment of genetic relationships among the chicken populations have been previously reported (Sharma et al, 2001).
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