Abstract
This is the first report to use electrospun nanofibers which could be of considerable interest to the development of new antibacterial compounds against certain species of bacteria affect plants in different ways as bacterial leaf spot (bacterial midrib rot), Pseudomonas cichorii and bacterial blight Dickeya dadantii (Erwinia chrysanthemi). Electrospun nylon-6/chitosan (nylon-6/Ch) nanofibers were obtained from formic acid as a single solvent. Surface modification of electrospun nylon-6/chitosan nanofibers was observed by soaking the mat in aqueous solu- tion of glycidyltrimethylammonium chloride (GTMAC) at room temperature overnight to give nylon-6/N-((2-hydroxy-3-trimethylammonium) propyl) chitosan chloride (nylon-6/HTCC). The morphological, structural and thermal properties of the nylon-6/chitosan nanofibers were stud- ied by field-emission scanning electron microscopy (FE-SEM), X-ray diffraction (XRD), Fourier transform-infrared (FT-IR) spectroscopy, and thermogravimetric analysis (TGA). Biological screening demonstrated that Nylon-6/HTCC mat exhibited high potential antibacterial activity against Pseudomonas cichorii and Dickeya dadantii (Erwinia chrysanthemi) on protein profile. Pseudomonas cichorii and Dickeya dadantii (Erwinia chrysanthemi) examined using SEM were totally deformed and exhibited severe destruction
Highlights
Bacterial pathogens employ diverse strategies to infect their host plants
Electrospun nylon-6/chitosan nanofibers mat was treated with excess glycidyltrimethylammonium chloride (GTMAC) in water with stirring for 24 h and the mat was washed with excess water and dried in hood at room temp
The antimicrobial agent was introduced via surface modification of the electrospun nylon-6/ Ch nanofibers by treatment in aqueous solution of GTMAC at room temperature
Summary
Bacterial pathogens employ diverse strategies to infect their host plants. Losses due to postharvest disease may occur at any time during postharvest handling, from harvest to consumption. The Gram-negative bacterium Dickeya dadantii (Erwinia chrysanthemi) is a phytopathogenic bacterium causing soft rot diseases on many crops. Controlling of Pseudomonas cichorii and Dickeya dadantii (Erwinia chrysanthemi) by Electrospun Nanofibers of Nylon-6/chitosan Blends portant economical impact on lettuce, celery and chrysanthemum crops [6]. Chitosan has a number of commercial and possible biomedical uses It can be used in agriculture as a seed treatment and biopesticide, helping plants to fight off bacterial infections [8]. The natural biocontrol active ingredients, chitin/chitosan, are found in the shells of crustaceans, such as lobsters, crabs, and shrimp, and many other organisms, including insects and bacteria It is one of the most abundant biodegradable materials in the world. The antibacterial activity of this new finding was demonstrated against Pseudomonas cichorii and Dickeya dadantii (Erwinia chrysanthemi) the main causative of several vegetable and cash crops diseases
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