Abstract
Genetic relationship between some species of Zea mays and Sorghum was determined using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of seed protein and random amplification of polymorphic DNA (RAPD-PCR) markers. According to SDS-PAGE analysis, 78 band were identified across the studied species. The number of bands varies from 17 bands in sample number 5 to 6 in sample number 6. Analysis of RAPD-PCR of DNA provided more precise information concerning relationships between Zea mays and Sorghum species than SDS-PAGE analysis. A remarkable result from this study was identifying a close relationship between Zea mays spp mays and Zea mays spp Mexicana. Further support comes from the molecular data of RAPD, which indicate that close relationship between Sorghum valgare and Sorghum bicolor. Key words: Zea mays, Sorgum volgare pres, Punciu milia ceaum L. protein, random amplification of polymorphic DNA.
Highlights
Phenotypic variation is positively associated with genetic diversity, but is dependent on environmental factors, as well as, on the interaction between genotypes and environment (Moose and Mumm, 2008)
The use of randomly amplified polymorphic DNA (RAPD) gave higher similarity coefficients than SDS-PAGE did for the same data, regardless of the higher number of polymorphic products generated by SDS-PAGE
RAPD-polymerase chain reaction (PCR) of DNA is effective in determining relationships between sections and subgenera
Summary
Phenotypic variation is positively associated with genetic diversity, but is dependent on environmental factors, as well as, on the interaction between genotypes and environment (Moose and Mumm, 2008). Maize (Zea mays L.) is one of the most diverse crop species, containing tremendous variation in morphological and physiological traits and extensive polymorphism in its DNA sequences. This exceptional diversity allows maize to be cultivated in a range of environments from temperate to tropical regions including parts of Africa, the Amazonian rainforest, Arizona deserts, the Gaspe Peninsula in Canada, and the Andes. As the closest wild relative of maize, teosinte, which is indigenous to Mexico and Central America Vollbrecht and Sigmon 2005, is a potentially important resource for the study of maize genetics and evolution and for plant breeding. Recently-developed molecular genetic techniques have provided another opportunity to assess the degree of genetic relatedness between maize and teosinte. We report the results of using two molecular techniques, randomly amplified polymorphic DNA (RAPD) of total genomic DNA and sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) of seed proteins
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