Abstract
Mycoplasma synoviae (MS) is one of the important pathogens in chicken and turkey which cause great economic losses in poultry industry. M. synoviae has one serotype but there is heterogeneity among MS strains. The aim of this study was to analyze the DNA sequence of Mycoplasma synoviae isolates from Mazandran province poultry flocks of Iran and compare them with MS from other countries. For this purpose, 32 samples were collected from choanal cleft, trachea and air sac of flocks with respiratory symptom and cultured in specific PPLO medium. Ten out of the 32 samples were positive in culture and reacted with specific antisera. The 10 positive samples were subsequently used for molecular study. The PCR products containing 16s rRNA genome were generated with specific primers and then sequenced. Multiple alignment and phylogenic analysis of DNA sequences was performed with DNASTAR program. Alignment and comparison of the sequences in DANSTAR program revealed that 7 of 10 isolate are quite similar and 3 other isolate are different from group one. Phylogenic analysis of M. synoviae isolated from Mazandran province and 21 M. synoviae from the entire world revealed low homology among most of them. However, some of the Mazandran isolates showed high homology with M. synoviaeisolated from Brasilia. Overall, these results revealed the different molecular structure and heterogeneity among M. synoviae isolated from Mazandran province in this study. Key words: Mycoplasma sinoviae, sequence, phylogenetic, poultry, Mazandran.
Highlights
Avian mycoplasmas occur in a variety of bird species.The most important mycoplasmas for chickens and turkeys are Mycoplasma gallisepticum (MG), Mycoplasma synoviae (MS) and Mycoplasma meleagridis
The Polymerase chain reaction (PCR) product containing 16s ribosomal RNA (rRNA) genome that was generated with specific primers and produced a specific 207 base pair band was sequenced (Figure 1) Alignment and comparison of the sequences in DNASTAR program revealed that 7 out of 10 isolates are quite similar and 3 other isolates are different from group one
Phylogenetic analysis of DNA sequences of M. synoviae isolated from Mazandran province of Iran and 21 M. synoviae from the entire world (Table 1) revealed low homology among most of them
Summary
Avian mycoplasmas occur in a variety of bird species.The most important mycoplasmas for chickens and turkeys are Mycoplasma gallisepticum (MG), Mycoplasma synoviae (MS) and Mycoplasma meleagridis. Mycoplasmas are bacteria that lack cell wall and belong to the class Mollicutes. They have been considered as extracellular agents, scientists admit nowadays that some of them are obligatory intracellular microorganisms, whereas all other mycoplasmas are considered facultative intracellular organisms (Kleven, 2003; Nasimento et al, 2005). M. synoviae (MS) infection most frequently occurs as a subclinical upper respiratory infection. It may cause air sac lesions when combined with Newcastle disease (ND), infectious bronchitis (IB) or both. MS becomes systemic and results in infectious synovitis, an acute to chronic infectious disease of chickens and turkeys, involving primarily the synovial membranes of Aliabad et al 2125 joints and tendon sheaths producing an exudative synovitis, tenovaginitis or bursitis (Kleven, 2003)
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