Abstract
Medicinal plants are potential sources of natural compounds with biological activities, and therefore attract the attention of researchers worldwide. The objective of this research is to determine the chemical composition of methanolic seed extract. The phytochemical compound screened by spectroscopy and gas chromatography-mass spectrometry (GC-MS) method. Sixteen bioactive phytochemical compounds were identified in the methanolic extract of Origanum vulgare. The identification of phytochemical compounds is based on the peak area, retention time molecular weight, molecular formula, MS Fragment- ions and pharmacological actions. GC-MS analysis of O. vulgare revealed the existence of the 1,7-Dioxaspiro[5,5]undec-2-ene, 2,4-Dihydroxy-2,5-dimethyl-39(2H)-furan-3-one, 2,4-Difurobenzene, 1-benzyloxy, α-D-Glucopyranoside, O-α- Glucopyranosyl, 4-Hexenal, 6-hydroxy-4-methyl,dimethyl acetal, acetate, 4H-pyran-4-one,2,3,-dihydro-3,5-dihydroxy-6-methyl, Benzofuran, 4-Amino-1,5-pentandioic acid, 2-Methoxy-4-vinylphenol, d-Mannose, 7-Isopropyl-10-methyl-1-oxo-1,5-dithia-spiro[5,5]undecane-2-carboxy, Phytol, Cis-Vaccenic acid, N-Methyl-Nbenzyltetradecanamine, 3,8,8-Trimethoxy-3-piperidyl-2,2-binaphthalene-1,1,4,4-tetrone, and 17-(1,5-Dimethylhexyl)10,13-dimethyl. The FTIR analysis of O. vulgare seeds proved the presence of alkenes, aliphatic fluoro compounds, alcohols, ethers, carboxlic acids, esters and hydrogen bonded alcohols, phenols. Key words: GC/MS, bioactive compounds, FT-IR, O. vulgare.
Highlights
Origanum vulgare L. is a perennial aromatic herb belonging to the family Lamiaceae (Skoula and Harborne, 2002) used for thousands of years as spices and as local medicines in traditional medicine (Altameme et al, 2015)
Aerial flowering parts of O. vulgare subsp. viride are used in Iranian traditional medicine as diuretic, stomachic, antineuralgic, antitussive and expectorant (Afsharypuor et al 1997)
The seeds were dried at room temperature for two weeks, and when properly dried powdered using clean pestle and mortar, and the powdered plant was size reduced with a sieve
Summary
Origanum vulgare L. is a perennial aromatic herb belonging to the family Lamiaceae (Skoula and Harborne, 2002) used for thousands of years as spices and as local medicines in traditional medicine (Altameme et al, 2015). O. vulgare L. is the most wide spread among all the species within the genus. It is distributed all over Europe, West and Central Asia up to Taiwan (Ietswaart, 1980; Hameed et al, 2015). Aerial flowering parts of O. vulgare subsp. Viride are used in Iranian traditional medicine as diuretic, stomachic, antineuralgic, antitussive and expectorant (Afsharypuor et al 1997).
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