Abstract
Six actinomycetes strains were isolated from soil and deteriorated wood taken from an oldhouse in the medina of Fez, Morocco. The antimicrobial activity of these strains wastested against Gram-positive bacteria (Bacillus strains and Staphylococcus strains) andGram-negative bacteria (Pseudomonas strain and Escherichia strain) and Candida albicans. Among the six isolates, five showed activity against at least one of the testmicroorganisms. The activity was also found in organic extracts of the isolates. Furthermore, the organic extract from one strain was sensitive to temperature and proteinase K indicating that the active substance would be of protein nature. Molecularidentification of the isolates was performed on the basis of 16S rDNA sequence analysis. Three isolates were attributed to Streptomyces genus, namely: Sj32 which could be identified as Streptomyces flavolimosus, Sj33 (Streptomyces spiroverticillatus) and Sj38 (Streptomyces parvus) whereas Sj68 and Sj69 could be indicated as Saccharothrixviolacea. Key words: Actinomycetes, Streptomyces, Saccharotrix, antimicrobial activity, molecular identification, 16S rDNA.
Highlights
The increasing emergence of resistant and multi-resistant bacteria worldwide and the lack of appropriate antibiotics to fight them continue to be the main concern of the medical community (Kuti et al, 2002)
Three isolates were attributed to Streptomyces genus, namely: Sj32 which could be identified as Streptomyces flavolimosus, Sj33 (Streptomyces spiroverticillatus) and Sj38 (Streptomyces parvus) whereas Sj68 and Sj69 could be indicated as Saccharothrix violacea
The target strains used for screening antimicrobial activity are: Gram-positive bacteria (Bacillus subtilis CIP 5262, Bacillus cereus CIP 14579, Staphylococcus aureus 7625, Staphylococcus epidermidis 6821), Gram-negative bacteria (Pseudomonas aeruginosa 76110, Escerichia coli CIP 7624) and the yeast Candida albicans
Summary
The increasing emergence of resistant and multi-resistant bacteria worldwide and the lack of appropriate antibiotics to fight them continue to be the main concern of the medical community (Kuti et al, 2002). The organic extract from one strain was sensitive to temperature and proteinase K indicating that the active substance would be of protein nature. We were interested in this study to carry out isolation and molecular identification of new strains of actinomycetes from an old house in the medina of Fez, Morocco and their screening for the production of Jihani et al 7371 antimicrobial substances.
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