Abstract
Engineering provitamin A synthesis pathway with β-carotene metabolism in rice endosperm of a restorer line BR827R
Highlights
Vitamin A is an essential vitamin for mammals
It has been established that four enzymes in plants, i.e. phytone synthase, phytone desaturase, ζ-carotene desaturase, and lycopene cyclase to complete the pathway toward β-carotene biosynthesis from GGPP
Peter Burkhardt (1996) reported while the plants were co transformed with plasmids pCPsyH and pCPdsH, both higher and expected size was observed
Summary
Vitamin A is an essential vitamin for mammals. Mammals are not able to produce carotenoids because of the complete absence of the biosynthetic pathway beginning with the synthesis of phytoene from GGPP. It has been established that four enzymes in plants, i.e. phytone synthase, phytone desaturase, ζ-carotene desaturase, and lycopene cyclase to complete the pathway toward β-carotene (provitmain A) biosynthesis from GGPP (for review see Britton, 1988; Cunningham and Grantt, 1998; Sandmann, 1994, 2001). Through biolistic method of gene transformation Phytone synthase (psy), bacterial phytone desaturase (crtI), and lycopene cyclase (lcy) genes were introduced into the endosperm of a restorer line to derive the accumulation of β-carotene. This is the initial report to develop a transgenic β-carotenoid restorer line, a new tool for improving hybrid rice
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