Abstract
In light of autofluorescence-free background, phosphorescent nanoprobes permit optical detection to effectively eliminate autofluorescence and scattering light through time-resolved luminescence methodology. To further amplify or boost target signals, phosphorescent nanoprobes are rationally fabricated to be “smart” or activatable, which also means only “turning on” at specific analytes. Maximizing the target signal while minimizing the background signal, phosphorescence-based activatable nanoprobes play a vital role to enhance sensitivity and selectivity for optical chemo/biosensing. Herein, we review the advanced development in time-resolved luminescent chemo/biosensing applications, and focusing on the fabrication of those phosphorescence-based activatable nanoprobes. Besides, it introduces some advanced long-lived emissive nanomaterials during the recent years. Finally, the current challenges and further perspectives are addressed to accelerate the advancement of phosphorescent nanoparticles in time-resolved optical sensing.
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