Abstract
Newcastle disease virus (NDV) causes heavy economic losses to poultry industry across the globe every year. Although NDV matrix (M) protein is involved in virus budding and our previous data indicate that in ovo expression of M protein facilitates NDV replication, the underlying mechanism for the role of M protein in NDV replication is not clear. Using yeast two-hybrid system and immunoprecipitation approaches, we found that M protein interacted with host vacuolar sorting protein charged multivesicular body protein (CHMP) 4B and 4C. In addition, the colocalization of M protein and CHMP4B/C could be observed using a laser confocal scanning microscope. Knockdown of CHMP4B by siRNA or transient expression of CHMP4B/C dominant negative forms markedly inhibited NDV growth in DF-1 cells. Thus, cellular CHMP4s play a critical role in NDV replication.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
