Abstract
Glucose- or glutamine-limited continuous cultivation of hybridoma cells was carried out. Under steady-state conditions, the specific consumption and production rates were calculated at various specific growth rates. On the basis of these results, the fluxes of the metabolic pathway were calculated. It was found that increasing the specific growth rate caused the specific ATP and antibody production rates to decrease. An estimation of the ATP consumption rates for cell growth and antibody production showed that the proportion of ATP consumed in antibody production decreased at a high specific growth rate. In order to increase the ATP production while keeping the specific growth rate high, the reduced metabolic fluxes were increased by the addition of pyruvate. As a result, higher specific ATP and antibody production rates were achieved at a high specific growth rate.
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