ENDURANCE AND INTOXICATION INDICATORS OF RAT BRAIN WITH CHRONIC ALCOHOLIZATION

Abstract

Introduction. One of the current important and unsolved problems of modern medicine is revealing the nature of the impact of chronic alcohol intoxication on functional indicators of life. It is important to analyze the metabolic shifts caused by ethanol, as well as to findeffectiveways to prevent and correct the effectsof alcohol. Aims. Research the effectof chronic ethanol consumption on endurance of rats, and determination of indicators of inflammation,intoxication and nonspecificresistance in the brain of laboratory rats. Materials and Methods. The experiment was performed on 2-month-old male and female rats. Alcohol intoxication was reproduced by introducing ethanol into drinking water, starting with 5% and gradually increasing to 15%. Intact groups of males and females received water. Endurance of rats was assessed once a week by the Porsolta Behavioral Despair Test, in which rats were given negative buoyancy by additional weight. The time of endurance of rats served as an indicator of fatigue. The duration of the experiment constituted 108 days. Inflammation(elastase, acid phosphatase activity), intoxication (urease activity and malonic dialdehyde activity), nonspecificresistance (lysozyme and catalase activity) were determined in the brain. Main results. It was found that the exercise stress contributes to the formation of more pronounced endurance of females than males. Chronic administration of ethanol did not significantlyaffectthe endurance of males during the swimming test, and led to a decrease in endurance of females. Biochemical studies showed that prolonged administration of ethanol to male and female rats leads to the accumulation of toxic products (urease, ammonia, lipid peroxidation products), stimulates the intensificationof inflammation(increased activity of acid phosphatase) against the suppression of nonspecificresistance (decrease of catalase and lysozyme activity) in the brain of rats under chronic alcohol intoxication. Conclusions. Alcohol consumption did not significantlyaffet the endurance of males, but reduced the endurance of females. The survey of the brain of rats showed the accumulation of toxic products, the intensificationof inflammationagainst the background of the suppression of nonspecificresistance under the influenceof ethanol.