Abstract
Insects have a long history of association with endosymbiont microbes. There are several indications that endosymbiont microbes are associated with insects and participate in the degradation of organic and inorganic molecules. This research aims to identify the profile of abundance and diversity of endosymbiont microbes associated with Nilaparvata lugens in Konawe (Southeast Sulawesi), Pasuruan (East Java), Klaten (Central Java), and Karawang (West Java) using a metagenomic study approach. We analyzed 15 pairs samples of N. lugens obtained from several regions in Indonesia. Endosymbionts were identified using a full-length primer 27F and 1492R targeting the 16S rRNA gene. The results showed that the proteobacteria phylum dominated all samples. Arsenophonus nasoniae (Morganellaceae; Enterobacterales) contributed the highest abundance (50-62%) based on total NumRead nucleotide base sequences from each sample. The bacterial diversity in these four samples was classified as moderate. This research showed that there were similarities in the communities and profiles of endosymbiont microbial constituents in each region, and this study becomes the basis for further research regarding the role of endosymbiont microbes on their hosts.
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