Endophytic Stenotrophomonas geniculata KJ-6 via producing antifungal volatile organic compounds effectively control Lanzhou lily postharvest diseases

Abstract

The endophytic bacterial Stenotrophomonas geniculata KJ-6, was isolated from Arisaematis rhizoma. This strain has the ability to control three postharvest pathogenic fungi of Lanzhou lily (Trichoderma lixii F-2, Talaromyces tumuli F-3, and Fusarium annulatum F-6) by releasing volatile organic compounds (VOCs). The experiment utilized the double-layer sealed bottom plate method. The results demonstrate that KJ-6 effectively inhibits the mycelial growth of F-2, F-3, and F-6, with inhibition rates of 81.59%, 79.87%, and 84.7%, respectively. Moreover, scanning electron microscopy (SEM) revealed abnormal mycelium morphology in the test pathogenic fungi treated with KJ-6 VOCs, characterized by multiple wrinkles, cracks, and distortions. Using five common phytopathogenic fungi as controls, our results demonstrated that the VOCs from KJ-6 exhibited broad-spectrum antifungal effects. Analysis using headspace gas chromatography-ion mobility spectrometry identified three components of the VOCs produced by KJ-6, including 1-propanethiol, isoamyl acetate, and 2-methyl-1-butanol. Antifungal tests revealed that 1-propanethiol plays a crucial role in the inhibitory activity of VOCs. It exhibited inhibitory rates of 64.17%, 72.73%, and 26.71% on the mycelium growth of F-2, F-3, and F-6, respectively. In vivo experiments further confirmed that VOCs can significantly reduce the disease index and decay rate of Lanzhou lily bulbs during storage. Moreover, KJ-6 treatment led to a significant reduction in the degree of browning of lily bulbs. Additionally, it enhanced the activity of defense-related enzymes, improved disease resistance, and extended the shelf life of lily bulbs. As a result, this study offers a theoretical foundation for the biological control of postharvest diseases in Lanzhou lily.