Abstract

This study aims to investigate the inhibitory effects of free and bound bioactive extracts from naked oat on amylase and glucosidase activity. The free compound extract mainly included kaempferol-3-O-galactoside-6″-rhamnoside-3‴-rha, hesperetin-7-O-neohesperidoside, ginsenoside Re and soyasaponin Ba. Ten substances were detected in the bound compound extract, including ferulic acid, oxyacanthine, and trans-4-coumaric acid. The free and bound compound extracts showed dose-dependent inhibitory effects on α-amylase and α-glucosidase; the inhibition of α-amylase was reversible anti-competitive inhibition, and the inhibition of α-glucosidase was reversible linear mixed inhibition. The saponin ginsenoside Re had poor inhibitory effects on α-amylase and α-glucosidase; the saponin soyasaponin Ba had a poor inhibitory effect on α-amylase but a good inhibitory effect on α-glucosidase. After adding the extracts to the different starches, the hydrolysis rates all decreased significantly (p < 0.05). This study elucidates the low digestibility of naked oats and will help facilitate the development of low-glycemic foods using naked oats.

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