Enantioselective, Potentiometric Membrane Electrodes Based on C70 Fullerenes for the Enantioanalysis of S-Clenbuterol in Serum

Abstract

Two enantioselective, potentiometric membrane electrodes (EPMEs) based on carbon paste impregnated with [5-6] fullerene-C70 (I) and diethyl (1,2-methanofullerene C70)-71-71-dicarboxylate (II) for the enantioanalysis of S-clenbuterol are reported. The electrodes exhibited near-Nernstian (higher than 55.00 mV/decade of concentration) responses only over S-clenbuterol in the following concentration ranges: 10−10 – 10−5 and 10−9 – 10−4 mol/L, for (I) and (II), respectively. The limits of detection were of 10−11 and 10−10 mol/L magnitude order, for EPMEs based on (I) and (II), respectively. The proposed electrodes proved to be successful for the enantioanalysis of S-Clenbuterol as raw material and from serum samples: average recovery of S-Clen in serum samples being higher than 98.40%, with RSD values not exceeding 1.25%.